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T7 terminator

Fig. S1. Pst I digestion + ligase. Pst I. Pst I. T7-TopRI-5. T7-TopRI-4. Pst I. Pst I. Pst I. Pst I. PCR 4 + 5. T T7. T T7. Bam HI. Bgl II. pET-9d. T7 terminator. T T7. P T7. ATG. pQET7-TopRI. megaprimer. T7-TopRI-3. Col E1. Sac I. Xho I. Sac I. Xho I. PCR 3 + mega.

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T7 terminator

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  1. Fig. S1 Pst I digestion + ligase Pst I Pst I T7-TopRI-5 T7-TopRI-4 Pst I Pst I Pst I Pst I PCR 4 + 5 TT7 TT7 Bam HI Bgl II pET-9d T7 terminator TT7 PT7 ATG pQET7-TopRI megaprimer T7-TopRI-3 Col E1 Sac I Xho I Sac I Xho I PCR 3 + mega ATG (7301 bp) ATG ATG ATG PT7 His6 topRI PT7 PT7 topRI His6 S.solfataricus topRI PCR 1 + 2 T7-TopRI-1 T7-TopRI-2 PT7 His6 topRI amp Xho I Sac I T7 promoter His-tag ATG PT5 / lacO ATG His6 topRI Xho I Sac I Sac I / Xho I digestion + ligase Xho I Sac I His-tag amp T5 promoter / lac operator element pQE31-TopRI Col E1 (7180 bp) S.solfataricus topRI Pst I

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