1 / 19

Chemolithotrophic proteobacteria S and Iron oxidizing

Chemolithotrophic proteobacteria S and Iron oxidizing. (SCIENCE 284: 16 April 1999) Thiomargarita namibiensis Beggiatoa (Winogradsky column) Thiothrix/Thioplaca Thiobacillus endosymbionts of deep-sea vent invertebrates. Quiz.

sebastian-makris
Download Presentation

Chemolithotrophic proteobacteria S and Iron oxidizing

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Chemolithotrophic proteobacteriaS and Iron oxidizing • (SCIENCE 284: 16 April 1999) Thiomargarita namibiensis • Beggiatoa (Winogradsky column) • Thiothrix/Thioplaca • Thiobacillus • endosymbionts of deep-sea vent invertebrates

  2. Quiz 1. Why is 16S rRNA such a good molecule for making phylogenetic trees (give 2 good reasons). (3) 2. What is the evolutionary distance between these two sequences. You may define it as a %. GGCCCTATATAC GCGCCTTTATAC (2) 3. Chloroplasts were originally _______, and are proof for theory of ________ (2) 4. Name three different ways to define a bacterial species (3)

  3. Nitrifiers • Nitrosonomas and Nitrobacter • Winogradsky

  4. Hydrogen oxidizers • Alcaligenes ”Knallgas reaction“ • 2 H2 + O2 ---- 2 H2O

  5. Methanotrophs and Methylotrophs • Not all methylotrophs are methanotrophs • Use methanol, methylamine, formate and (methane) • Type 1 (ribulose monophosphate pathway) and II (serine pathway), membranes differ too • Type 1 membranes are throughout cell • Type II on periphery of cell • Endosymbionts in deep-sea vent mussels

  6. Photosynthetic proteobacteria • Purple phototrophic proteobacteria (bacteriochlorophylls and photophosphorylation) • Purple sulfur, eg. Chromatium • Below is Chromatium tempedium from Yellowstone, that grows above 50C. Notice the phase-bright sulfur granules

  7. Purple non-sulfur (photoheterotrophs) • (other groups in the Bacteria that photosynthesize?, green sulfur, green filamentous, cyanobacteria)

  8. Spirilla: • Spirillum _some magnetotactic e.g. Magnetospirillum p 487 • Bdellovibrio, attack gram – bacteria, see developmental cycle

  9. Sulfate/sulfur reducers • Desulfurovibrio, Desulfuromonoas, anoxic habitats, compete with methanogens • use, H2, lactate as e'donors and sulfate or sulfur as e'acceptor. What is the product?

  10. Budding and stalked • Hyphomicrobium (methylotroph), budding • Caulobacter, stalked, see cycle

  11. Sheathed Proteobacteria: • e.g Leptothrix • Mn 2+ + 0.5 O2 + H2O --- Mn O2 + 2H+ delta G –68kJ

  12. Fermentative rods and vibrios 1.Enterics (gamma) • Gram -, nonsporulating, facultative aerobes, fermentative, opportunistic pathogens • 2 types: mixed acid fermenters (produce acid) E. coli OR 2,3-butanediol fermenters (produce neutral products) Enterobacter aerogenes/Erwinia Escherichia Salmonella typhi- typhoid fever Serratia marcescens (Bright red pigment- prodigiosin) Xenorhabdus (bioluminescence) Klebsiella pneumoniae (not normally pathogenic)

  13. Vibrio, Photobacterium • V. cholerae • Photobacterium • O2 required for luciferase activity • Quorum sensing • FMNH2 + O2 +RCHO ---- FMN +RCOOH + H2O + hv • Need flavin mononucleotide, aliphatic aldehyde, O2, and enzyme. E- donor is NADH

  14. Oxidative rods and cocci • Pseudomonads (human pathogens, biodegraders, plant pathogens) • Azotobacter and free-living N-fixers • Rhizobium and Bradyrhizobium: symbiotic N-fixers • Agrobacterium, crown gall • Acetobacter and Gluconobacter • Legionella, • Neisseria • Rickettsia obligate parasite, Rocky mountain spotted fever

More Related