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CRTI 06-0230 RD: Rapid Methods for Emergency Bioassay

CRTI 06-0230 RD: Rapid Methods for Emergency Bioassay. Chunsheng Li, Health Canada Gary Kramer, Health Canada Baki Sadi, Health Canada Diana Wilkinson, DRDC-Ottawa Kui Yu, National Research Council Yi Cui, National Research Council Edward Lai, Carleton University

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CRTI 06-0230 RD: Rapid Methods for Emergency Bioassay

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  1. CRTI 06-0230 RD: Rapid Methods for Emergency Bioassay Chunsheng Li, Health Canada Gary Kramer, Health Canada Baki Sadi, Health Canada Diana Wilkinson, DRDC-Ottawa Kui Yu, National Research Council Yi Cui, National Research Council Edward Lai, Carleton University Amy Hrdina, Carleton University Negar Bahraini, Carleton University

  2. Project Introduction • Project Background - Rapid bioassay is required to screen large population - Po-210 and Sr-90 are two high risk but “difficult” radionuclides • Design Objectives - Detection Limit: 10% of derived urine action level (Bq/L) based on 500 mSv committed effective dose equivalent from inhalation - Urine throughput: 120 samples in the first 48 hours per system - Feces throughput: 20 samples in the first 48 hours per system

  3. Project Tasks • Developing rapid methods for measuring Po-210 and Sr-90 in bioassay samples using “traditional approach” - Based on currently available techniques - Focusing on rapidness, sensitivity, accuracy, repeatability, and field deployability etc - Studying the metabolism of Po-210 • Exploring the application of new materials and new technology to radiation assessment - Application of quantum dots in radiation measurement - Application of magnetic nano-parrticles in sample preparation

  4. Figure 4: This spectrum shows yttrium-90 growth. From top to bottom: no yttrium, yttrium growth after 1 hour, 10 hours, and 96 hours respectively. Spectra from liquid scintillation counter Tri-Carb 3180 TR/SL. Achievements To Date (I):Rapid Method for Sr-90 Bioassay • R

  5. Urine sample Acidification & Transferring (5 minute) Preconditioning & decolourization (10 minutes) Complexation & pH adjustment (5 minutes) Anion exchange separation (15 minutes) Cocktail mixing & Liquid scintillation measurement (15 minutes)

  6. Baki, Sadi, Chunsheng Li, Sara Jodayree, Vera Kochermin, Edward Lai, Gary Kramer “A rapid bioassay method for the determination of Sr-90 in human urine samples”. Submitted to Health Physics, 2009 Chunsheng Li, Baki Sadi, Gerry Moodie, Joseph daka, Edward Lai, Gary Kramer “Field deployable Technique for Sr-90 emergency bioassay”. Sumitted to Radiation Protection Dosimetry, 2009

  7. ZnSe CdTe 400 600 800 1000 1200 1400 HgS CdSe HgTe CdS InP InAs Emission Wavelength (nm) Achievements To Date (II):Application of QDs

  8. A Robert Z Stodilka, Jeffrey JL Carson, Kui Yu, Md. Badruz Zaman, Chunsheng Li, and Diana Wilkinson “Optical Degradation of CdSe/ZnS Quantum Dots upon Gamma-Ray Irradiation”, J. Phys. Chem. C. 2009, 113,2580-2585.

  9. Yu, Kui; Cui, Yi; Zaman, Md. Badruz; Wilkins, Ruth; Li, Chunsheng; Wu, Xiaohua; Ouyang, Jianying; “Optical Response of CdSe Quantum Dots to Cs-137 γ Radiation” J. Nanoscience andNanotechnology. 2009, Accepted.

  10. Achievements To Date (III):Application of MNPs in Bioassay

  11. Magnetic particles NIP-PMAA-coated magnetic particles NIP-coated magnetic particles

  12. Achievements To Date (IV):Metabolism of Po-210 in Rats • Primary: volatile Po-210 species in excreta - Implicating the accuracy in radiation dose assessment - Implicating in radiation protection - Providing information for bioassay method development • Secondary: IR imaging – change in thermal profile for invasive screening • Secondary: excretion and bio-kinetics - Excretion via urine - Excretion via feces - Distributions in tissues/organs

  13. THE END

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