1 / 12

Electrochemical Detection in HPLC

Electrochemical Detection in HPLC. ALEXYS ™ - HPLC/ECD. Applications Neuroscience Pharma Clinical analysis Food & Beverages Environmental. Why ECD?. extremely sensitive highly selective . Principle of HPLC/ECD. electrochemical reaction detector potential (E) is driving force.

senwe
Download Presentation

Electrochemical Detection in HPLC

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Electrochemical Detection in HPLC

  2. ALEXYS™ - HPLC/ECD • Applications • Neuroscience • Pharma • Clinical analysis • Food & Beverages • Environmental

  3. Why ECD? • extremely sensitive • highly selective

  4. Principle of HPLC/ECD • electrochemical reaction detector • potential (E) is driving force

  5. Electrochemical reaction ΔE diffusion electron transfer reaction

  6. 100 80 60 Signal (%) 40 20 0 0 400 800 1200 1600 E (mV) Role of electrode potential E • Facilitates the EC reaction oxidation occurs no reaction A  B

  7. Working range potential E • E working range determined by mobile phase

  8. Peak height vs. concentration • UV  Beer’s law E =  d C • ECD  Cottrell equation ilim= 1.47 n F (Fm )1/3 (DA/b)2/3 C A= WE area A b = spacer thickness C = concentration ilim= diffusion limited current measured (peak height) peak height (E) vs concentration (C) peak height (Ilim) vs concentration (C)

  9. Peak height vs. concentration • UV  Beer’s law E =  d C • ECD  Cottrell equation ilim= f (A/b)2/3 C A= WE area A b = spacer thickness C = concentration ilim= peak height as simple as abc…

  10. Electroactive Groups

  11. Application areas… www.myAntec.com

  12. Summary Electrochemical Detection for HPLC • Potential driven • Selective • Sensitive Thank you!

More Related