1 / 15

HPLC

HPLC. High Performance Liquid Chromatography. WJEC Pathways Applied Science Unit 2.8 WJEC GCSE Additional Applied Science. Diagram HPLC. solvent reservoir. pump . This is where separation occurs. detector. HPLC column. HPLC Syring e. injection port. waste . Testing for drugs.

silas
Download Presentation

HPLC

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. HPLC High Performance Liquid Chromatography WJEC Pathways Applied Science Unit 2.8 WJEC GCSE Additional Applied Science

  2. Diagram HPLC solvent reservoir pump This is where separation occurs detector HPLC column HPLC Syringe injection port waste

  3. Testing for drugs • HPLC can be used to test for ‘performance enhancing’ drugs that may be used by athletes

  4. What are we looking for? Aspirin • Some endurance athletes have been drugs such as aspirin before and during competition in an attempt to compete at the highest intensity for the longest duration • Aspirin is also taken to relieve symptoms of colds etc • Not on the International Olympic Committee (IOC) banned list Caffeine • Caffeine is also used athletes as a performance aid • The IOC presently lists caffeine as a banned substance Amphetamine • These may mean an athlete feels less tied and can endure longer • All forms of amphetamines are bannedby the IOC Urea • Naturally formed in the body

  5. What is in the sample?Who has broken the rules? • Identify what is present in each sample of blood taken from three different athletes. • All samples were taken under the same conditions

  6. Choose the sample to run • Aspirin • Caffeine • amphetamine • Urea • Athlete A • Athlete B • Athlete C • Athlete A -overlay Conditions of analysis Temperature: 25 oC Mobile phase: 50% water 50% acetonitrile Flow rate: 2 cm3 /min Column: C-18 Analyst: Ann Lyst

  7. Aspirin Return to menu 0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 Time (minutes)

  8. Caffeine Return to menu 0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 Time (minutes)

  9. Amphetamine Return to menu 0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 Time (minutes)

  10. Urea Return to menu 0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 Time (minutes)

  11. Athlete A Return to menu 0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 Time (minutes)

  12. Athlete B Return to menu 0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 Time (minutes)

  13. Athlete C Return to menu 0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 Time (minutes)

  14. aspirin caffeine urea amphetamine Athlete A and overlay Return to menu 0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 Time (minutes)

More Related