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Hands-on Chromatography

Learn about chromatography, a technique used to separate similar compounds based on their adsorption strength. Explore various types of chromatography and hands-on activities to practice the method. Suitable for beginners and students.

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Hands-on Chromatography

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  1. Hands-on Chromatography Sheryl K. Hoffmann Concordia College CONASTA 60

  2. Definition 1: Chromatography is a technique for separating similar compounds from one another by using differences in the strength of their adsorption on an inert material. Adsorb: hold (molecules of a gas or liquid or solute) to its surface Absorb: swallow up, incorporate, take in Definition 2: Chromatography is a technique which can be used to separate the components of a mixture. The method depends upon differences in the abilities of the components to adhere to a porus solid such as silica gel, called the stationary phase differences in the abilities of the components to be dissolved in a stream of liquid or gas, called the mobile phase, which is passed over the solid. Definitions

  3. Main Types • Paper Chromatography • Thin Layer Chromatography (TLC) • Column Chromatography • High Performance Liquid Chromatography (HPLC) • Gas-Liquid Chromatography

  4. Basic Method

  5. Spotting Technique

  6. Tips • If the spot is large, components start from different origins and will thus blur any separation that would normally occur. • Try a quick dabbing motion, the longer the liquid is in contact with the paper the larger the spot • If the spot is not very concentrated the resulting bands, once spread over a wider area, will be even fainter. • If the solution to be spotted is not very strong, spot several times, being sure to allow the spot to dry in-between, a hair drier will speed up the drying process.

  7. Hands On Activities 1. Practice Spotting 2. Comparison of 1M NaCl & Water in Separating Food Colour 3. Sticks of Chalk 4. Counterfeit Smarties? 5. Comparing Black Inks

  8. Activity 2 – Food Dyes

  9. Activity 3 - Chalk

  10. Activity 4 - Smarties

  11. Activity 5 – Black Pen Ink

  12. Activity 6 – Geranium TLC Paper

  13. Activity 6A – Frozen Spinach

  14. Activity 6A –Spinach

  15. Activity 7 – 2 Columns

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