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160. NKL/HepG2. NKL/HepG2+IFN- . 140. 120. N.S. N.S. 100. 80. Cytotoxicity%. 60. 40. 20. 0. 10:1. 5:1. E:T.
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160 NKL/HepG2 NKL/HepG2+IFN- 140 120 N.S. N.S. 100 80 Cytotoxicity% 60 40 20 0 10:1 5:1 E:T Fig S1.Cytotoxicity of NKL cells against IFN--treated HCC cells.HepG2 cells was pretreated with 200pg/ml IFN- for 6 h, and then exposed to parental NKL cells for cytotoxicity assay after washing off the IFN-. Data shown are means±SD from at least three separate experiments. *P<0.05, **P<0.01 versus NKL-vec cells.
HepG2/NKL-vec HepG2/NKL-IFN HLA-ABC Fig S2.Expression of HLA-ABC on HepG2 cells. HepG2 cells were co-cultured with NKL-IFNor NKL-vec cells by transwell method. After 24 h, the expression levels of HLA-ABC in HepG2 cells were determined by FACS.One representative of at least three independent experiments.