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Yes, You Can Do It! … A Practical Approach to ABO Discrepancies

Yes, You Can Do It! … A Practical Approach to ABO Discrepancies. Cynthia Steinmetz, MT(ASCP) Lead Reference Technologist LifeShare Blood Centers. ABO Discrepancies. Definition Essential Patient Information Common Sources of Technical Errors

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Yes, You Can Do It! … A Practical Approach to ABO Discrepancies

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  1. Yes, You Can Do It! … A Practical Approach to ABO Discrepancies Cynthia Steinmetz, MT(ASCP) Lead Reference Technologist LifeShare Blood Centers

  2. ABO Discrepancies • Definition • Essential Patient Information • Common Sources of Technical Errors • Discrepancies Divided into 4 Groups 2

  3. Group I Discrepancies • Unexpected reactions in serum/plasma reverse grouping • Weakly reacting or missing antibodies • More common than in other groups • Populations with discrepancies in this group 3

  4. Group II Discrepancies • Unexpected reactions in red cell forward typing due to weakly reactive or missing antigens • Causes of discrepancies in this group • Resolution of Group II discrepancies 4

  5. Group III Discrepancies • Forward and reverse groupings affected • Protein and plasma abnormalities resulting in rouleaux formation • Causes; disease associations • Resolution of Group III discrepancies 5

  6. Group IV Discrepancies • Forward and reverse typing discrepancies due to miscellaneous problems • Causes • Resolution of Group IV discrepancies 6

  7. Group IV Discrepancies Case Studies 7

  8. Case 1 • OB patient • Age 19 • Specimen submitted as ABO discrepancy.

  9. Case 1 - Blood Typing

  10. Case 1 - Additional Testing

  11. Case 2 • 84 year old woman • Anemia • No history of transfusion

  12. Case 2 - Blood Typing

  13. Case 3 • 28 year old OB patient

  14. Case 3 - Blood Typing

  15. Case 3 - Antibody Screen

  16. Case 3 - Panel

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