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UCL. Proteomic, M etabolomic and L ipidomic Research Capability at ICH and ION. Lipidomics. UCL. Proteomic, M etabolomic and L ipidomic Research Capability at ICH and ION. Same principle as the proteomic mass spectral method
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UCL Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION Lipidomics
UCL Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION • Same principle as the proteomic mass spectral method • Urine/plasma/CSF injected into a UPLC QTOF mass spectrometer • lipids separated by UPC2 chromatography (supercritical fluid chromatography) • Uses CO2 to separate lipids out according to their polar properties not non-polar (reverse phase) • Lipids are easier to identify than metabolites as they have been more extensively studied C22 C20 C18 C24 C16 C14 C12 Free fatty acids C10 C8
UCL Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION • Same principle as the proteomic mass spectral method • Urine/plasma/CSF injected into a UPLC QTOF mass spectrometer • lipids separated by UPC2 chromatography (supercritical fluid chromatography) • Uses CO2 to separate lipids out according to their polar properties not non-polar (reverse phase) • Lipids are easier to identify than metabolites as they have been more extensively studied Triacylglycerols Chosteryl esters ( Mixture of FFA, TAG, and CE
UCL C34:0 Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION • Phospholipid analyses from human heart C32:0 Phosphatidylcholine C36:0 C30:1 C36:1 Phosphatidylethanolamine Phosphatidylcholine Sphingomyelin C36:3 C38:3 ceramides Lyso-phosphatidyl- ethanolamine Lysophosphatidyl choline Phosphatidylglycerol