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Phase 2: The Calvin Cycle. a.k.a The Dark Reactions. Products of Phase 1 are used in Phase 2. ATP Energy NADPH e-(electrons). Stage 2: Calvin Cycle. Purpose: Make sugar by reducing carbon dioxide (adding e-) Location: Stroma of chloroplast. Stage 2: Calvin Cycle.
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Phase 2: The Calvin Cycle • a.k.a The Dark Reactions
Products of Phase 1 are used in Phase 2 • ATP Energy • NADPH e-(electrons)
Stage 2: Calvin Cycle • Purpose: • Make sugar by reducing carbon dioxide (adding e-) • Location: • Stroma of chloroplast
Stage 2: Calvin Cycle • Requires: ATP* • Electrons (NADPH)* • CO2 • Enzymes
Stage 2: Calvin Cycle • Produces • Glucose
A brief note about Rubisco • Enzyme that attaches CO2 to 5-carbon compound in Calvin cycle • Most abundant protein on the earth! • Rate limiting – a slow enzyme (~ 3 / sec.) • Genetic engineers trying to make a more efficient Rubisco
Calvin Cycle • CO2 enters cycle by becoming attached to a 5-Carbon RuBP • produces 6-carbon molecule (unstable) • Breaks into two 3-carbon molecules
Calvin Cycle • Energy is used (ATP ADP) • Electrons used (NADPH NADP+) • 3-Carbon compound leaves Calvin cycle
Calvin Cycle • Two 3-Carbon groups combine to become glucose • Remaining 3-Carbon groups continue through cycle to regenerate starting 5-Carbon RuBP • Six CO2 to produce 1 glucose(6 turns of the cycle required)
Important molecules are recycled • ATP ADP • NADPH NADP+ • 5-Carbon RuBPrecycles
Photosynthesis Summary Input: Light Water CO2
Photosynthesis Summary • Output: • O2 • Glucose
Photosynthesis Summary Recycle: ATP Electron carriers 5-Carbon RuBP