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Control Positive

25. HLA-A/GUSB. 20. 15. 10. 5. 0. E-004. E-159. Control positive + IFN- g. E-159 + IFN- g. E-004 + IFN- g. A. Real-time PCR. 40. HLA-B/GUSB. 35. 30. 25. HLA-A mRNA copy number/ GUSB mRNA copy number. HLA-B mRNA copy number/ GUSB mRNA copy number. 20. 15. 10. 5. 0.

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Control Positive

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  1. 25 HLA-A/GUSB 20 15 10 5 0 E-004 E-159 Control positive + IFN-g E-159 + IFN-g E-004 + IFN- g A Real-time PCR 40 HLA-B/GUSB 35 30 25 HLA-A mRNA copy number/ GUSB mRNA copy number HLA-B mRNA copy number/ GUSB mRNA copy number 20 15 10 5 0 E-004 E-159 E-159 + IFN-g Control Positive Positive Control + IFN-g E-004 + IFN- g Positive Control B IFN-g Flow Cytometry IFN-a HLA-ABC Negative control HLA-b2m HLA-ABC HLA-b2m ESTDAB-004 counts counts counts Constitutive expression HLA-ABC HLA-b2m HLA-b2m HLA-ABC counts counts counts Inducible expression ESTDAB-159 FL1-Height FL1-Height FL1-Height FL1-Height

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