Presentation Transcript

1. Nuts and Spices

   By: Ashley, Kristen, Chealsa, and Melinda
2. Objective Extract oils from both walnuts and hazelnuts and compare IR and GC-MS results to the standards Extract oil from nutmeg and caraway seeds and compare the IR and GC-MS data to that of the standards, eugenol, limonene, and carvone.
3. Importance of Experiment To develop a good extraction technique for the essential oils of nuts and spices. The SFE was a good choice of instrumentation because it is one step and is more green for the environment. Nut oils are used primarily for cooking and there is a huge market for them. Spice essential oils are used for fragrance and can be sold for other purposes.
4. Procedure SFE We used a range from 2-5g of sample masses Ran each sample twice Massed extracted samples Ran product using IR and GS-MS
5. Procedure (Continued) FTIR was used for all standards and extracted products except that from caraway seed GC-MS 12 drops of analyte were dissolved in 15mL of hexane and ran on the instrument
6. Data SFE extracted products Day 2: 0.4403g hazelnut oil (2.0615g nuts) Day 3: 0.7285g hazelnut oil (5.0136g nuts) 0.6921g hazelnut oil (4.7657g nuts) Day 4: 0.8113g walnut oil (4.5077g nuts) 0.5656g walnut oil (4.5877g nuts) Day 5: 0.5757g nutmeg oil (4.5113g nutmeg) 0.9846g nutmeg oil (4.5088g nutmeg) Day 6: 0.0748g ? (4.4244g caraway seeds) 0.0274g ? (4.6563g caraway seeds)
7. Data FTIR Nutmeg oil has all expected peaks of the literature value Eugenol matches literature value but does not match the extracted product Extracted walnut oil is identical to the standard with all of the same principal peaks Extracted hazelnut oil is identical to the standard with all of the same principal peaks
8. Data (GC-MS) Walnut Standard Hazelnut Standard Hazelnut Sample
9. Data (GC-MS) Eugenol Standard Limonene Standard
10. Error SFE product Too low of mass resulted in not enough product Nutmeg Was a waxy solid with a yellow Not actually eugenol (IR spectrum) Caraway Seeds Unable to crush with a mortar and pestle (crack) Not a an oil or a solid Unable to run FTIR of GC-MS on product Cannot conclude what was extracted
11. Error (continued) Walnut Sample Carvone standard
12. Problems Timing issues Correcting the SFE So many people using the SFE so it needed extra cleaning Leading to 2 samples a day instead of 3 IR Amount of sample we had available to run GC-MS The instrument itself during the second week no longer held pressure and required daily checks and could only run 1 sample a day these are the error samples
13. Conclusion Overall Hazelnut and Walnut oils were very successful matching up to the IR spectras Nutmeg did not match up to the expected standard but did after looking at our results we found it matched nutmeg oil. Unexpected Success Caraway Seeds were overall unsuccessful and we did not have enough time to analyze our product due to issues with the GC-MS
14. Further Possibilities We could run other nuts since the two we ran were so successful. We could develop a better technique for the extraction of caraway seeds. In the future, the process for determining the best technique for each spice or nut could be worked out. We could also do further research and determine if essential oils can be extracted from any other foods or products.
15. Thanks to… Amanda =] Oh, SFE Problems