1 / 26

TOPIC : Microscope AIM : How do we use a microscope?

TOPIC : Microscope AIM : How do we use a microscope?. Total magnification. Eyepiece lens magnification x objective lens magnification. Total magnification =4 x10= 40. 100x. 400x. Rules when using a microscope.

sharonmccormick
Download Presentation

TOPIC : Microscope AIM : How do we use a microscope?

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. TOPIC: MicroscopeAIM: How do we use a microscope?

  2. Total magnification Eyepiece lens magnification x objective lens magnification

  3. Total magnification=4 x10= 40

  4. 100x 400x

  5. Rules when using a microscope 1. Carrying microscope = 1 hand on arm, other hand under base close to body

  6. 2. DO NOT use coarse adjustment under high power bc it can break the lens or the slide

  7. 3. Always start off with low power bc it gives you a greater field of view. LOW HIGH

  8. 4. Before switching to high power you must: a. focus your image b. center your image

  9. 5. Adjust the diaphragm (When switching from low to high, amount of light decreases)

  10. 6. Use LENS PAPER to clean lens

  11. What is staining? Treating specimen with solutions of different colors Make cell parts more visible

  12. Unstained cheek cells Stained cheek cells

  13. Examples: 1. Lugol’s Iodine (plant cells)

  14. Onion Cells stained with Lugol’s Iodine

  15. 2. Methylene blue (animal cells)

  16. Cheek Cells Unstained Stained

  17. Skin Cells stained with Methylene Blue

  18. Wet mount 1. Place a drop of water on the slide

  19. 2. Place object on the slide.

  20. 3. Slowly lower cover slip onto specimen on an angle to avoid AIR BUBBLES.

  21. 4. Absorbed excess water with a paper towel.

  22. How do images appear? Upside down & backwards On the slide Seen through lens F

  23. What happens when you move the slide? Image moves in opposite direction

More Related