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ERT314 BIOREACTOR SYSTEM

ERT314 BIOREACTOR SYSTEM. CHAPTER 2: BIOLOGICAL SYSTEMS AND MEDIA DESIGN. 2.1 Introduction to Biological System. ** rheology – the study of the flow of matters, especially in liquid state. Fermentation with fungi. Fermentation with bacteria. Oxygen Demand. OTR = Oxygen transfer rate

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ERT314 BIOREACTOR SYSTEM

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  1. ERT314 BIOREACTOR SYSTEM CHAPTER 2: BIOLOGICAL SYSTEMS AND MEDIA DESIGN

  2. 2.1 Introduction to Biological System

  3. **rheology – the study of the flow of matters, especially in liquid state

  4. Fermentation with fungi Fermentation with bacteria

  5. Oxygen Demand • OTR = Oxygen transfer rate • OUR = Oxygen uptake rate

  6. Most organisms reduce the pH during growth.

  7. LET’S RECALL…

  8. E.Coli has a system where the capacity to use these precursors exceed the capasity of intermediary metabolism e.g: glycoprotein, oligosaccharides, enzyme etc.

  9. 2.2.1 Microbial ProcessesA. Design and Formulation of Media Should consider:

  10. e.g: e.g: antagonism (Zn+ to Cu2+), synergism (toxic Cu2+ + Cd2+), stimulation (Ca2+ and Mn2+ depends on Zn2+)

  11. requires higher pantothenic acid content in media Addition of glycine betaine in media for L- amino acid production which has high glucose concentration, acts as an osmotic protectant.

  12. (if more than 10 mM, no antibiotic is synthesized)

  13. (easily altered)

  14. causing Whereas others, like dried yeast are generally uniform from batch to batch.

  15. General equation for the stoichiometry of growth and product formation

  16. Where yx/s, yp/s and yCO2/s are = coefficients related to biomasss, product and CO2 formation = expressed as C mole of biomass, product and CO2 per mole of carbon and energy source (substrate) σi = carbon fraction of i (x,p,s) Yx/s = g dry weight of biomass per g of substrate consumed Yp/s = g dry weight of product per g of substrate consumed

  17. Constraints: • Low reactor rated and productivity • Instability of cell lines presents problem not easy to overcome • Danger of microbial contamination • Secondary metabolites is often retained in biomass • Plant cells are often shear sensitive • In some cases, secondary compounds should be removed rapidly How to overcome?

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