3500

Aug 30, 2014

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ko3. A. A. Prom. ko1. ko5. 0. 3500. dbpB. dbpA. ko4. ko2. A. Prom. A. ko1. ko5. pGEMT-dbpBA-AscI. dbpB. dbpA. ko4. kan3’. A. A. P flgB- kan. kan5’. kan3’. Prom. ko1. ko5. A. A. dbpB. pKHdbpBAko. P flgB- kan. dbpA. ko4. kan5’. comp1. ko5. comp3. Prom. dbpA.

sheng

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3500

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ko3 A A Prom ko1 ko5 0 3500 dbpB dbpA ko4 ko2 A Prom A ko1 ko5 pGEMT-dbpBA-AscI dbpB dbpA ko4 kan3’ A A PflgB-kan kan5’ kan3’ Prom ko1 ko5 A A dbpB pKHdbpBAko PflgB-kan dbpA ko4 kan5’ comp1 ko5 comp3 Prom dbpA pKH2000 comp4 comp2 comp5 C B ko5 + kan5’ kan5’ + kan3’ FlaB 5’ + FlaB 3’ BbKH500 Bb297 BbKH500 Bb297 M 1 2 3 4 5 6 M - - + + AscI 2500 2500 2000 2000 1500 1500 1000 1000 700 700 500 500 250 250