1 / 37

Chapter 10 Gene Isolation and Manipulation

Chapter 10 Gene Isolation and Manipulation. Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse. . Finding specific nucleic acids by using gel electrophoresis and blotting. Polymerase chain reaction (PCR). Sequencing.

sheng
Download Presentation

Chapter 10 Gene Isolation and Manipulation

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Chapter 10 Gene Isolation and Manipulation Microinjection of foreign DNA into a 1-cell mouse embryo resulting in a transgenic mouse.

  2. Finding specific nucleic acids by using gel electrophoresis and blotting

  3. Polymerase chain reaction (PCR)

  4. Sequencing

  5. Sanger Sequencing (Dideoxy Sequencing) The structure of 2’,3’-dideoxynucleotides

  6. The dideoxy sequencing method

  7. Reading the DNA sequence from an automatic sequencer

  8. How to amplify an interesting gene

  9. Making a cDNA clone

  10. Introduction of a transgene

  11. Agrobacterium tumefaciens causal agent of Crown Gall

  12. The Ti plasmid vector

  13. The generation of a transgenic plant

  14. The generation of a transgenic plant

  15. Pattern of transmission of T-DNA

More Related