simple affordable complete

1. simple affordable complete Brant Bassam PhD www.corbettlifescience.com

2. Easy to use system • Lowest possible cost per extraction • Hands-free, walk-away operation • High speed + high throughput (96 well) • Consolidated chemistryminimum reagents and kit types work for all sample types • Complete integrated system solutionautomation, reagents, consumables, protocols, options

3. 770 mm open Work surface enclosed by acrylic hood Dual position Manifold 1. Vacuum to Waste 2. Vacuum to Elute 2 2 1 1 Tip discard chute 390 mm closed 8-Channelpipetting head 4 SBS plate positions 3–8 Reagent Tubs(variable volumes) 650 mm 490 mm 25 kg Bench Top Unit

4. To X-tractor Gene manifold Vacuum chamber coupling Pump coupling Bottle full sensor 520 mm High power pump Carbon exhaust filter Waste bottle4 L sanitary quick disconnect bottle PolyCap pump filter Vacuum sensor Software regulated -5 to -70 kPa, 80 L/min 495 mm 400 mm 19 kg Under BenchVacuum/Waste Station

5. Instrument Options UV Light • Irradiates surfaces between runs • Robot arm moves so light reaches all areas • Software controlled • Timed post-run operation • Must be factory fitted HEPA filter• Positive pressure clean air supply • Software controlled • Integrated design • Easy filter change • Can be added after install

6. Easy-to-use Wizard For standard protocols simply: 1. Click-select the columns to extract(in columns of 8) 2. Press Jump to End button and start run!

7. Software Intuitive color-coded interface mirrors actual deck layout Active screen prompts (where appropriate) For each reagent tub position, volumes needed are calculated for the number of samples to be extracted

8. Barcode Reader Compatible Sample names and information can also be imported or exported

9. Pre- and Post-Run Reports • HTML report (universal format) • Provides details of a run • Documentation & Traceability(e.g. sample, batch and lot identifiers) • Can be directly: • Printed • Saved • E-mailed • Exported to Word

10. Bind-Elute Protocol

11. PDF Protocol (Instructions for Use) X-tractor Gene Run File CorProtocols™ for different applications • Available for web download • Each comprises:

12. Coming Soon: • Viral Kit(VX Reagent Pack) CorProtocol™ Reagents DNA Extraction: P/N 2220 DX Reagent Pack P/N 2228 DXT (Tissue Digest Reagent)

13. DX Reagents • Abbreviations aid recognition (DXL, DXB, etc) DX Reagent Pack (P/N2220) comprises: 1. DXL Liquid Digest Reagent P/N2221 2. – DX Digest Enzyme P/N2222 3. – DX Binding Additive P/N2224 5. DXB Binding Reagent P/N2223 4. DXW Wash Reagent P/N2225 6. DXF Final Wash Reagent P/N2226 7. E Elution Reagent P/N2227 DXT Tissue Digest Reagent P/N2228 (Reagent is for separate purchase if required)

14. X-tractorConsumable Pack(standard) P/N 2270 Sufficient for 480 Extractions 60 x Elution Plate 8-Strip Caps 960 x OnCor™ C 200 µL Tips 5 x Square-well Lysis Blocks 5 x Capture Plates 5 x Elution Plates(96 plain tube cluster rack) 5 x Sealing Films

15. Lysis Plate Square well design for maximum capacity (2.2 mL/well) P/N 2271 96 Square-well Lysis Plate

16. Capture Plate Standard Plate ~10 µg capacity P/N 0913 Capture Plate, Low Skirt, 800µL

17. Elution Plate Custom designed rack for X-tractor Gene Convenient format Grid-referenced lid design Tab-lock on lid Cluster rack design(separate tubes) P/N 2147 Elution Plates, 96 Plain Tube Cluster Rack

18. Seals P/N 2280 Sealing Film P/N 1636 Elution Plate 8-Strip Caps Silicon press-on type Grid referenced (A–H) Seals and protects any unused portion of a capture plate

19. Fully pocketed rack design • Separate pockets for each tip prevents cross-contamination, allowing tip re-use during a run • Tip re-use reduces costs • Uses only 1.5 tip racks per 96 extractions OnCor™ C Tips rack design • SBS rack with replaceable lid for easy removal and storage between runs • Total rack enclosure for security and contamination resistance P/N 2299 OnCor™ C 200 µL Fine Bore Tip 96 tips/rack, 10 racks/box

20. OnCor™ C Design • Best liquid clearance height • Optimized barrel and taper • Improved filter matrix • High-Set Filter Design • Better liquid clearance height • Less wetting issues OnCor™ C Tips design evolution • Unmodified tip • Filter set too low • Easily wet and blocked

21. OnCor™ C Tips Advanced robotic tip design exclusive to Corbett Robotics High-Set filter guards against filter wetting Capp™ compatible hub dimensions (“C” tips) Wide upper barrel for greater liquid capacity and clearance between the liquid and filter inside the tip Larger filter and new more effective matrix Optimized terminal taper minimizes sample immersion and displacement effects Low retention polymerfor improved liquid shedding and pipetting precision Fine bore tip for precision liquid handling P/N 2299 OnCor™ C 200 µL Fine Bore Tip 96 tips/rack, 10 racks/box

22. clearance height with 200 µL liquid OnCor™ C Tips improved liquid clearance height High-Set Filter OnCor™ C Unmodified tip Example wet filter

23. Disposable Reagent Tubs • Convenient • Streamlines setup • Graduated for volume (mL) • Reusable lids curb evaporation • Extremely low reagent dead volume(e.g. 0.75 mL in a 70 mL tub) P/N 2137 70 mL size P/N 2136 170 mL size P/N 2314 270 mL size

24. CorProtocol™ Options

25. Options High Yield Capture Plate ~40 µg capacity P/N 1863 High Yield Capture Plate

26. Cap options: Grid referenced tubes in racks with individual Twist-Lock caps* Silicon 8-strip capsP/N 1636 Label options: • Grid Reference* • 2D Cipher Barcode* • Plain Unlabelled(P/N 2147 in purple rack, not shown) H9 Tube Options Volumes: 0.65 mL Standard tubes 0.5 mL Twist-Lock tubes *from ABgene or authorized distributor only

27. 3rd Party Chemistry Options Accessories are available to use a wide range of kits Please note Corbett Robotics cannot provide support for reagents and plasticware of other manufacturers

28. Sample Digest OptionVortemp 56 Shaking Incubator • Can improve speed & completeness of digest • Variable vortex speed—200 to 1500 rpm • Broad temperature range to 100°C • Interchangeable platforms for Microtubes and Microplates

29. 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G H DX Reagent Verification Testing Experimental setup Reagents and Consumables DX Reagent Pack (P/N2220) was functionally tested using Whole Blood CorProtocol™ standard X-tractor Consumable Pack (standard; P/N 2270). Samples • Whole bovine blood was collected in an EDTA Vaccutainer • Maximum whole blood sample size was tested (200 µL) • 96 samples total were processed (whole 96-well plate);48 whole blood and 48 no template controls (NTCs) • To test for well-to-well cross contamination, samples were extracted in a checkerboard pattern (as shown) alternating positive samples (●) and NTCs (●).

30. 40 Kb 40 Kb 20 Kb 20 Kb 10 Kb 10 Kb 6 Kb 6 Kb 4 Kb 4 Kb 3 Kb 3 Kb 2 Kb 2 Kb 1 Kb 1 Kb DX Reagent Verification Testing cont. Gel electrophoresis of raw extracted DNA from positive samples: reproducible yields of high quality, intact high molecular weight DNA (~40 kb) G2 MW B1 D1 F1 H1 A2 C2 E2 B3 D3 F3 H3 A4 C4 E4 G4 B5 D5 F5 H5 A6 C6 E6 G6 MW MW B7 D7 F7 H7 A8 C8 E8 G8 B9 D9 F9 H9 A10 C10 E10 G10 B11 D11 F11 H11 A12 C12 E12 G12 MW

31. DNA Concentration ng/µL 260/280 Ratio (from 180 µL whole blood eluted in 150 µL) 90 2.000 80 1.800 70 60 1.600 50 ng/µL DNA 1.400 40 30 1.200 20 260/280 Ratio 1.000 10 0.800 0 0 8 16 24 32 40 48 0.600 Sample # 0.400 0.200 0.000 0 8 16 24 32 40 48 Sample # DX Reagent Verification Testing cont. Spectrophotometer analysis of all positive samples Note: the results shown are indicative only, actual yields will vary depending on the species and the health of the individual from which blood was harvested.

32. DX Reagent Verification Run ■Positive samples ■ NTC samples Maximum CT 25.04 Minimum CT 22.19 Average CT 24.59 CT Std. Dev. 0.18 DX Reagent Verification Testing cont. Real-time amplification analysis of all samples Real-Time Analysis Real-Time quantitative amplification analysis was done on all samples targeting the G6PD1 gene to single copy sensitivity (40 cycles) to test for inhibitors and for evidence of cross-contamination Amplification Results • early, reproducible amp. of all positive samples • no evidence of PCR inhibitors Cross-contamination Results • no amplification of alternate NTC samples • no evidence of cross-contamination Using the X-tractor Gene™ will minimise any lab’s contamination risk!

33. Some sample types successfully extracted • Blood • whole, fresh, frozen, buffy coat • Human, Mouse, Turtle, Bovine… • Serum/Plasma, HIV, Hep B , Hep C, CMV • Urine • Chlamydia and N. gonorrhoea • Swabs • HSV1 and 2 • Tissue • Mouse Tails • Prawn Tissue • Bird Quill Pulp • Nasal Aspirates • MPV (a single stranded RNA Virus) • Cultured cells • Micro Organisms Bacteria (Gram Pos. and Gram Neg.) and Fungi

34. Benefits • Consistency & Workflow • Reduce human error and free-up technician time • Standardisation • Between labs and runs • Documentation • Pre- and Post-Run reports • Safety • No phenol/chloroform • All waste safely contained for disposal • Harmful fumes are carbon filtered out • Work area completely enclosed by hood • Run pauses if the hood is opened

35. Summary • Complete-Solution • Instrument, reagents, consumables, protocols • Substantial Cost Reduction • About half other methods • Minimum Possible Cross-Contamination Risk • Optimized liquid handling strategies • High-Throughput • 96 samples in about 1.5 hours • Versatility and Choice • Compatible with a wide range of protocols and 3rd party chemistries

36. Gene-Disc™ 100 96-well Workflow Integration

37. Offices Brisbane Australia Corbett Robotics Pty Ltd 42 McKechnie Drive Eight Mile Plains, QLD 4113 T +61 7 3841 7077 F +61 7 3841 6077 United Kingdom Corbett Research UK Ltd Unit 1 Terek House, Sandpiper Court Phoenix Business Park, Eaton Socon St Neots, Cambridgeshire, PE19 8EP T +44 (0) 1480 407222 F +44 (0) 1480 407999 Sydney Australia Corbett Research Pty Ltd 14 Hilly Street Mortlake, NSW 2137 T +61 2 9736 1320 F +61 2 9736 1364 USA Corbett Robotics Inc 185 Berry Street, Suite 5200 San Francisco, CA 94107 USA T +1 415 348 1166 F +1 415 348 1177 E-mail info@corbettlifescience.com Web www.corbettlifescience.com All slides  2006 Corbett Life Science. All rights reserved