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Memory & PKMζ

Memory & PKMζ. Biochemistry 658 Elizabeth Saunders. PKm ζ. Protein Kinase M zeta Constitutively active isoform of Protein Kinase C (PKC) Functions in the storage of memory but not acquisition of memory.

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Memory & PKMζ

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  1. Memory & PKMζ Biochemistry 658 Elizabeth Saunders

  2. PKmζ Protein Kinase M zeta Constitutively active isoform of Protein Kinase C (PKC) Functions in the storage of memory but not acquisition of memory. Long Term Potentiation (LTP): a lasting increase in a neuron’s ability to send more frequent signals to adjacent neurons.

  3. ZIP & AMPAR Pseudosubstrate ζ-Inhibitory Peptide (ZIP): prevents PKMζ from binding to AMPAR. Α-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid receptor (AMPAR): transmembrane glutamate receptor.

  4. Cohen Study Will localized injections of ZIP disrupt traumatic memory formation and stress response in rats? Predator Scent Stress (PSS) Paradigm. • Experimental group exposed to soiled kitty litter. • Control group exposed to unsoiled kitty litter. • Measure of stress response: freezing in place. Study 1: injections of ZIP and Scr-ZIP locally given immediately after PSS exposure. Study 2: injections of ZIP and Scr-ZIP locally given 10 days after PSS exposure. The ability of all rats to respond to PSS control post ZIP and Scr-Zip injections.

  5. Four brain Structures Parts of the brain implicated in memory processes and anxiety Dorsal Hippocampus (DH) Lateral Ventricle (LV) Basolateral Amygdala (BLA) Insular Cortex (IC)

  6. What happened? Study 1: immediate local injections • DH & LV: fear abolished • BLA & IC: anxiety observed Study 2: injection 10 days post injection • IC: fear abolished • BLA, LV & DH: anxiety observed Conclusion: ZIP successfully abolished the traumatic memory of the PSS.

  7. Applications Post Traumatic Stress Disorder (PTSD) • ZIP may work to reduce anxiety/remove anxiety resulting from traumatic memories by erasure. Neurological Pain Disorders • ZIP may disrupt pain signaling in nervous tissues.

  8. Sources 1. Sacktor T. How does PKM? maintain long-term memory?. Nature Reviews Neuroscience [serial online]. January 2011;12(1):9-15. Available from: Academic Search Premier, Ipswich, MA. With permission from Todd C. Sacktor  2. Cohen H, Kozlovsky N, Matar M, Kaplan Z, Zohar J. Mapping the brain pathways of traumatic memory: Inactivation of protein kinase M zeta in different brain regions disrupts traumatic memory processes and attenuates traumatic stress responses in rats. European Neuropsychopharmacology [serial online]. April 2010;20(4):253-271. Available from: Academic Search Premier, Ipswich, MA. With permission from Hagit Cohen

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