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Yuno Lee

Adenosine Kinase. Yuno Lee. adenosine. Nucleoside = adenine + ribose - β-N 9 -glycosidic bond. Energy transfer - adenosine triphosphate (ATP) - adenosine diphosphate (ADP) Signal transduction as cAMP. AK(adenosine kinase). Function

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Yuno Lee

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  1. Adenosine Kinase Yuno Lee

  2. adenosine • Nucleoside = adenine + ribose - β-N9-glycosidic bond. • Energy transfer - adenosine triphosphate (ATP) - adenosine diphosphate (ADP) • Signal transduction as cAMP.

  3. AK(adenosine kinase) • Function • ①ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. ②potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. • Subunit • monomer (by similarity). • Catalytic Activity • ATP + adenosine = ADP + AMP. • Cofactor • binds 3 magnesium ions per subunit (by similarity).

  4. AK(adenosine kinase) • Enzyme Regulation • activity is inhibited by 5-iodotubercidin and 5'-amino-5'-deoxyadenosine (by similarity). • Pathway • purine salvage. • Tissue Specificity • highest expression in liver, testis, kidney and spleen. lower expression in brain. isoform long is predominant in testis and kidney and isoform short is predominant in the spleen. • Similarity • belongs to the carbohydrate kinase pfkb family.

  5. FRONT BACK

  6. ATP binding site ADO binding site

  7. ATP binding site ADO binding site

  8. Topology diagram of AK. The -helices (labeled 1- 12) and the -strands (labeled 1- 14).

  9. ADO binding site

  10. ADO binding site

  11. ATP binding site

  12. ATP binding site

  13. Stereodrawing of the adenosine 1 binding site. • W:water(no tryptophan in here) , F:Phe, T:Thr, Q:Gln, D:Asp, N:Asn, G:Gly

  14. (b) Stereodrawing of the adenosine 2 binding site. W:water(no tryptophan in here) , T:Thr, Q:Gln, D:Asp, G:Gly, H:His

  15. Stereodiagram showing key active site residues and modeled (a) ATP and (b) Ap4A binding sites.

  16. Stereodiagram showing key active site residues and modeled (a) ATP and (b) Ap4A binding sites.

  17. References 1. Biochemistry,37 (45), 15607 -15620, 1998 Structure of Human Adenosine Kinase at 1.5 Å Resolution

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