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DNA EXTRACTION VIDEO

DNA EXTRACTION VIDEO. Video creation and design By Michael Cochran-Boucher And Jane Russell Special Thanks: Megan Boucher (Editing) Source: Utah.edu/Home DNA Extraction Lab. First, find a source of DNA, such as: Spinach Strawberries Broccoli Examples of DNA not to use, such as:

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DNA EXTRACTION VIDEO

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  1. DNA EXTRACTION VIDEO Video creation and design By Michael Cochran-Boucher And Jane Russell Special Thanks: Megan Boucher (Editing) Source: Utah.edu/Home DNA Extraction Lab

  2. First, find a source of DNA, such as: • Spinach • Strawberries • Broccoli • Examples of DNA not to use, such as: • Your family pet, Fido the dog • Your little sister’s big toe • Bugs caught in the yard

  3. Video part : 1

  4. Start: Step 1 Pause video: to read instructions • Set up the blender!!! • Add ½ cup of split peas (100ml) • Add 1 cup of water (200ml) • Add 1/8th teaspoon table salt • Blend for 30 seconds on Puree • Separate pea-cell soup • Pour mixture through strainer into glass container (discard strained solid peas) • Add 30ml liquid detergent to mixture and swirl • Let stand for 5-10 minutes • Pour Mixture into small glass containers (about a third full)

  5. Video part : 2

  6. Step 2 • Add the ENZYMES (virtual protein scissors), such as: • Meat tenderizer (a pinch to each container) • Pineapple juice • Contact lens cleaning solution • Stir each container gently(30-60 seconds) • Stirring to hard may break up the DNA strands making them harder to see

  7. Video part : 3

  8. Step 3 • Alcohol Separation: • Tilt test tube and slowly pour rubbing alcohol down the side • Watch for a layer to form on top • Pour until you have equal amounts: pea mixture and alcohol • Take wooden stick and slowly twirl the stick near top of mixture • Your DNA isolate should pull from the solution.

  9. Video part : 4

  10. Finish: Step 4 What is that Stringy Stuff? • “DNA is a long, stringy molecule. The salt that you added in step one helps it stick together. So what you see are clumps of tangled DNA molecules!”“DNA normally stays dissolved in water, but when salty DNA comes in contact with alcohol it becomes undissolved. This is called precipitation. The physical force of the DNA clumping together as it precipitates pulls more strands along with it as it rises into the alcohol.”““You can use a wooden stick or a straw to collect the DNA. If you want to save your DNA, you can transfer it to a small container filled with alcohol.” • Source: Utah.edu

  11. Video part : 5

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