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UWRF Gomashi Annotation

UWRF Gomashi Annotation. Matt Sutter Gabby Field Megan Kreitzman. Annotations. Table 3 genes 18-27 Gabby Field 18-20, Matt Sutter 21-23, Megan Kreitzman 24-27 All genes FWD and similar to Halo or Liefie. Pham Map Genes 18-27. Gomashi -draft Hope Liefie Halo.

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UWRF Gomashi Annotation

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  1. UWRF GomashiAnnotation Matt Sutter Gabby Field Megan Kreitzman

  2. Annotations • Table 3 genes 18-27 • Gabby Field 18-20, Matt Sutter 21-23, Megan Kreitzman 24-27 • All genes FWD and similar to Halo or Liefie

  3. Pham Map Genes 18-27 • Gomashi-draft • Hope • Liefie • Halo • Our map matches perfectly with the maps of other G phages. No genes are missing or added in this clip of genes. • Our section of genes enable Gomashi to attach to hosts.

  4. Confusion/Debate • Class -1 debate • LO- Listing length of longest • Good collaboration All C.P. included? LO? Multiple starts? Write annotation Good SD score Better blast match? OK overlap Change start = no = yes

  5. Gene 18 • Start Changed! • Possible start @ 16,650 SD score of 462 matches Liefiegp18 1:1 • Called start @ 16,641-18,404 (FWD) SD score of 378 and matches Liefiegp18 4:1 • Phamily: 4102, members: 38 • Pham shared with K1-K5 • Function: Minor tail protein

  6. Gene 19 • Possible start @ 18,359 with an overlap of 44, LO, SD score of 294 and a blast of 16:1 • Called start @ 18,404-18,865 (FWD) best SD score of 609, overlap of 1 bp, matches Halo gp19 1:1 • Phamily: 1333, members: 32 • Pham shared with K1-K5 • NKF

  7. Gene 20 • Possible start @ 18,658 with an overlap of 206 • Called start @ 18,862-19,998 (FWD) best SD score of 525, gap of 2 bp, matches Halo gp20 1:1 • Phamily: 3204, members: 33 • Pham shared with K1-K5 • Function: Minor tail protein.

  8. Gene 21 • One plausible start. Called @ 20,009-20,443 (FWD) good SD score of 483, overlap of 1, is LO, and 1:1 match with Halo gp21 • 48.1 % threshold shows that gene most likely originated from cluster K • phamily: 4430 members: 32 • Pham shared with K1-K5 • NKF

  9. Gene 22 • One plausible start called @ 20,442-22,836 (FWD) good SD score of 462, is LO, matches Halo gp22 1:1 • Phamily: 3208, members: 12 • Pham unique to G • NKF

  10. Gene 23 • One plausible start called @ 22,851-23,171 (FWD) good SD score of 735, L.O, matches Halo gp23 1:1 • Pham: 1371, members: 12 • Pham unique to G • NKF

  11. Gene 24 • Possible start @ 23,168 matches Halo 3:1 • Called start @ 23,174-23,356 (FWD) good SD score of 378, gap of 2, matches Halo gp24 1:1 • Phamily: 1372, members: 12 • Pham unique to G • NKF

  12. Gene 25 • Possible start @ 23,353 matches Halo 33:32, 38 bp overlap • Called start @ 23,356-23,631 (FWD) best SD score of 525, overlap of 1, matches 32:32 with Halo • Phamily: 1373, members: 12 • Pham unique to G • NKF

  13. Gene 26 • Possible start @ 23,643 may not include all c.p., matches Halo gp26 1:5. • Called start @ 23,631-23,825 (FWD) includes all c.p. is L.O, good SD score of 441, matches Halo gp26 1:1 • Phamily: 3209, members: 12 • Pham unique to G • NKF

  14. Gene 27 • Possible start @ 23,871 with a SD score of 180. • Called start @ 23,886-25,202 (FWD) better SD score of 378, matches Halo gp27 1:1 • Phamily: 4284, members: 423 • Pham shared with A1-A10, B1-B5, C, D, E, F1, F2, H1, H2, I1, I2, J, K1-K5, L1-L3, M, N, O, P, Q, R, S, and T • Function Lys A

  15. Similar G Locations • Gomashi-River Falls, Wisconsin • Halo- Pittsburgh, Pennsylvania • LieFie- Durban, South Africa • BPs- Pittsburgh, Pennsylvania • Hope- Atlanta, Georgia

  16. Impact • Gene 18 change • Few G phages are sequenced • Annotation Experience • Thank you!

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