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The genetic map of bacteriophage l

The genetic map of bacteriophage l. Control of transcription in bacteriophage l life cycle by the anti-terminators N and Q proteins, the activator C II protein and the repressor/activator C I protein. Characteristics of diploid with gal80, gal4, and GAL81c mutations.

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The genetic map of bacteriophage l

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  1. The genetic map of bacteriophage l

  2. Control of transcription in bacteriophage l life cycle by the anti-terminators N and Q proteins, the activator CII protein and the repressor/activator CI protein

  3. Characteristics of diploid with gal80, gal4, and GAL81c mutations

  4. The steps and enzymes involved in the utilization of the sugar galactose in the yeast Saccharomyces

  5. The transcriptional orientation of the 3 genes coding for enzymes important in galactose utilization in Saccharomyces There synthesis is regulated by the transcription activator Gal4 protein.

  6. GAL4 bound to DNA

  7. A protein with a C6-zinc finger (involves 6 cysteines) Many transcription regulator proteins have one (or more) zinc-finger domains

  8. A retrovirus genome showing the location of the transcription activation sites (enhancers) The genome structure of mouse mammary tumor virus is shown here

  9. Analysis of genetic regulation using reporter gene constructs

  10. A transcription activator protein binds to the enhancer site and also interacts with components of the RNA polymerase to achieve increased transcription Enhancers and enhancer-binding proteins activate transcription reminiscent of the CAP site and CRP activator protein in the lac operon of E. coli.

  11. A model for the structure of activator proteins bound to 2 enhancers and RNA polymerase II bound to the promoter and the interactions between them Structures like this involving DNA with bound activator proteins and RNA polymerase complex are names “enhanceosomes”. TBP stands for TATA-binding protein, a component of RNA polymerase II associated factor, TFIID

  12. Uncovering of transcription protein binding sites by chromatin remodeling complexes makes binding by transcription-proteins possible

  13. Use of alternative promoters at different stages in life

  14. Different promoters may be enhanced depending upon which activator protein is present in a cell

  15. Alternative splicing of the primary transcript

  16. Structure of an immunoglobulin G (IgG) molecule

  17. The distribution of variable, joining and constant sequences which are spliced to create many different light chain proteins

  18. Mating type switching during the life cycle of some strains of Saccharomyces

  19. Both mating type genes are located on chromosome III of Saccharomyces. The mating type of the cellis determined by the sequence present at the MAT site

  20. Regulation of a-specific, a-specific and haploid-specific genes in Saccharomyces Three proteins (a1, a1 and a2) are involved in regulating the expression of these 3 classes of genes.

  21. Cutting by methylcytosine sensitive/insensitive restriction nucleases can be used to estimate the extent of cytosine methylation in a DNA sequence

  22. Imprinted genes in mammals

  23. Some human diseases are due to loss of sites involved in genomic imprinting

  24. Alternative splicing of mRNA

  25. Nonsense-mediated decay of mRNA

  26. Alt.splicing combined with NMD can be used for genetic control

  27. RNAi (RNA interference): dsRNA directs degradation of mRNA with the same/complementary sequence

  28. Translational control

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