1 / 33

STREPTOCOCCI

STREPTOCOCCI. Streptococci facultative anaerobe Gram-positive chains or pairs catalase negative (staphylococci are catalase positive). Streptococcus in chains. Streptococcus pneumoniae (diplococcus). Fluorescent stain. Streptococci Lancefield groups

signe-harding
Download Presentation

STREPTOCOCCI

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. STREPTOCOCCI

  2. Streptococci • facultative anaerobe • Gram-positive • chains or pairs • catalase negative • (staphylococci are catalase positive)

  3. Streptococcus in chains

  4. Streptococcus pneumoniae (diplococcus). Fluorescent stain

  5. Streptococci • Lancefield groups • Satu atau lebih spesies per group • antigen permukaan

  6. groupablestreptococci • A, B and D • most important • C, G, F • rare

  7. Non-groupable • S. pneumoniae • pneumonia • viridans streptococci • e.g. S. mutans • dental caries

  8. hemolysis reaction - sheep blood agar • α (alpha) • partial hemolysis • green color • β (beta) • complete clearing • A and B • γ (gamma) • no lysis

  9. Hemolysis • Groups A an B • β • Group D • α or γ • S. pneumoniae and viridans • γ

  10. hemolysis reaction + one characteristic • presumptive identification

  11. Group A streptococcus(S. pyogenes)

  12. Group A streptococcal menginfeksi semua umur • Insiden tertinggi pd usia 5-15 thn

  13. S. pyogenes -suppurative • non-invasive • pharyngitis • Infeksikulit, impetigo • invasive bacteremia • toxic shock-like syndrome • "flesh eating" bacteria • pyrogenic toxin

  14. Pyrogenic toxin • superantigen • T cell mitogen • Mengaktifkan sistem imun

  15. Scarlet fever • rash • erythrogenic toxin

  16. non-suppurative • rheumatic fever • inflammatory disease • life threatening • Sekuel kronik • demam • jantung • sendi • rheumatic tidak sama dengan rheumatoid arthritis

  17. Rheumatic fever -etiology • M protein • cross-reacts heart myosin • autoimun • Antigen dinding sel • sedikit dicerna in vivo • bertahan lama

  18. Rheumatic fever • penicillin • menyembuhkan pharyngitis • Menurunkan resiko carditis

  19. Acute glomerulonephritis • immune complex disease of kidney

  20. Major pathogenesis factors • lipoteichoic acid/F protein • fimbriae • binds to epithelial cells • M protein • anti-phagocytic

  21. M protein • major target • Imunitas alami • strain variation • antigenicity • re-infection • Terjadi dengan strain yg berbeda

  22. Capsules • Anti-phagocytic • mucoid strains

  23. Isolation and identification • β hemolytic colonies • bacitracin menghambat pertumbuhan • β hemolytic colonies • group A antigen

  24. β hemolysis • hemolysin O • Sensitif oksigen • hemolysin S • Non sensitive oksigen

  25. Post-infectious diagnosis (serology) • Antibodi thd streptolysin O • Penting! Bila menetap sekuel kronik terjadi

  26. Serotyping • M • T • R

  27. Group B streptococcus • neonatal meningitis • septicemia • transmission • vaginal flora

  28. Group B streptococcus - identification • β hemolysis • hippurate hydrolysis • CAMP reaction • meningkatkan βhemolisis S. aureus

  29. Group D streptococcus • Tumbuhpada bile esculin agar • precipitasihitam 6.5% salin • Tumbuh • enterococci • Tidaktumbuh • non-enterococci

  30. Enterococci • Hubungannya agak jauh dr streptococci lain • genus Enterococcus • Flora usus • urinary tract infection • Kontaminasi dr anus • opportunistic infections • khususnya endocarditis • Paling sering E. (S.) faecalis

  31. Enterococci • resisten thd banyak antibiotik • termasuk vancomycin • ujung D-ala diganti dengan D-lactat

  32. Minute colony streptococci • Various groups (e.g. group A) • berbeda scr genetik • koloni yg besar (e.g. S. pyogenes) • tdk ada demam rheuma Koloni besar Koloni kecil

  33. Viridans streptococci • diverse species • oral • Karies gigi • α hemolytic dan negatif thd tes lain • non-groupable. • termasuk S. mutans • endocarditis • cabut gigi

More Related