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Actuator. Input. Input. Head Receiver. Receiver/ Transmitter 1: ToxR. Assemble: J07027,J07028 prom::rbs::toxR::MalE/PhoA. BioBrick Pho A: J07005 MalE: J07005. Receive Brick ToxR: J07009. Can Fluor. Bind to fusion?. Does fluor. bind ⇨⇨ dimerization?. Is Fusion Complex
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Actuator Input Input Head Receiver Receiver/ Transmitter 1: ToxR Assemble: J07027,J07028 prom::rbs::toxR::MalE/PhoA BioBrick Pho A: J07005 MalE: J07005 Receive Brick ToxR: J07009 Can Fluor. Bind to fusion? Does fluor. bind ⇨⇨ dimerization? Is Fusion Complex Being Expressed? Where Is Fusion Complex Being Expressed? Assemble: R0040::B0030 prom::rbs Assemble: J07024-26 prom::rbs::toxR::scfv BioBrick anti-fluor scFV J070012, J070013, J070014 Test expression of scFV prom::J070012, J070013, J070014 Fluorescein dimer binds to scFV? Fluorescein ⇨ Cells? Fluorescein dimer ⇨Cells? Actuator: ctx::gfp J07011 Fluorescein Binding ⇨⇨ signal? Receive Brick ctx: J07007 Dimerization ⇨⇨ signal?