1 / 12

Neurophysiological and behavioral effects of mycotoxin ( patulin ) in albino mice

Neurophysiological and behavioral effects of mycotoxin ( patulin ) in albino mice. Al- Hazmi , N.A.*; El- Sayed Fahim ** and Al- Hazmi , M.A.*** * King Abdul-Aziz Univ ., Teachers collage ** Cairo Univ .,Faculty of Science , Zoology Dept.

sinjin
Download Presentation

Neurophysiological and behavioral effects of mycotoxin ( patulin ) in albino mice

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Neurophysiological and behavioral effects of mycotoxin (patulin) in albino mice Al-Hazmi, N.A.*; El-SayedFahim** and Al-Hazmi, M.A.*** * King Abdul-Aziz Univ., Teachers collage ** Cairo Univ.,Faculty of Science , Zoology Dept. *** King Abdul-Aziz Univ., Faculty of Science, Biology Dept.

  2. INTRODUCTION Patulin (4-hydroxy-4H-furo[3,2-c]pyran-2(6H)-one) is a toxic secondary metabolite produced by a wide range of fungal species of the Penicillium, Aspergillus, Byssochlamys, Eupenicillium and Paecilomycesgenera of which Penicillium expansum, a common contaminant of damaged fruits is the most important (Fuchs et al., 2008).

  3. Penicillium expansum, commonly identified as the ‘‘blue mold rot”, causes the most common storage rot of apples and is known to invade not only fruit but also other foodstuff such as vegetable , berries, bread and meat products (Rychlik & Schieberle, 2001; Ritieni, 2003). For humans, the major potential dietary sources of patulin (PAT) are apples and apple juices made from affected fruit (Murillo-Arbizu et al., 2009).

  4. MATERIALS AND METHODS Animals used in the study were the male albino MF1 mice brought from the center of King Fahad for research in King Abdul-Aziz university, their ages ranged from 10-12 weeks. To study the neurotransmitters, animals were divided into 2 group contained 30 male albino mice and 6 animals were decapitated each week. Group 1 : Control group animals were daily oral administered with saline (0.9 % NaCl) for 6 weeks. Group 2 : animals were daily oral administered with contaminated apple juice with patulin (152.5 µg/ 1ml) for 6 weeks.

  5. To study the behaviors of the animals, the animals were divided into 2 groups each group contain 10 animals. Group 1: To study Tube Restraint test for the aggressive behavior according to (Al-Hazmiand Brain 1991; Al-Hazmi, 2001) and the following parameters were calculated a- Proportion of animals biting the target . b- The latency period. And c- Number of bites. Group 2: To study locomotors activity test. This test aims to evaluate the difference in the behavioral activity of the animals according to the methods of Al-Hazmi and Brain,1991 ; Al-Hazmiet al., 1997, and the following parameters were calculated : A- Latency period. B- Time of locomotors. C- Time of immobility. D- Number of rear. E- Number of wall rear and F- Number of squares crossed.

  6. Neurotransmitters measurement: Dopamine (DA), norepinephrine(NE) and serotonin (5-HT) were estimated and determined according to the method of Chang, 1964 modified by Ciarlone 1978, by spectroflorometric method (Tyrnerquantech Tm, Digital filter, BransteadThermoline, Corporation, Asuvsidlary of Sybaon, International Company, U.S.A.). Brain areas were dissected according to the method of Glowinski and Iversen, 1966, into 7 regions which are : Cerebellum, Pons plus medulla oblongata, Striatum, Cortex, Hypothalamus, Midbrain and Hippocampus.

  7. Table (1): Effect of chronic oral administration of apple juice contaminated with patulin (152.5 µg/1ml) on dopamine (DA) content in the different brain areas of albino mice. n.s. non-significant change according to Student,s T-test. * significant change ( P< 0.05 ) according to Student,s T-test. ** highly significant change ( p< 0.01 ) according to Student,s T-test. *** more highly significant change ( p<0.001 ) according to Student,s T-test.

  8. Table (2): Effect of oral administration of apple juice contaminated with patulin (152.5 µg/ 1ml) on norepinephrine (NE) content in the different brain areas of albino mice. n.s. non-significant change according to Student,s T-test. * significant change ( P< 0.05 ) according to Student,s T-test. ** highly significant change ( p< 0.01 ) according to Student,s T-test. *** more highly significant change ( p<0.001 ) according to Student,s T-test. .

  9. Table (3): Effect of oral administration of apple juice contaminated with le patulin (152.5 µg/ 1ml) on serotonin (5-HT) content in the different brain areas of albino mice. n.s. non-significant change according to Student,s T-test. * significant change ( P< 0.05 ) according to Student,s T-test. ** highly significant change ( p< 0.01 ) according to Student,s T-test. *** more highly significant change ( p<0.001 ) according to Student,s T-test.

  10. Table (4): Explain (Range ± Median) on the effect of apple juice contaminated with patulin (152.5 µg/1ml) on the locmotors behavior of albino mice. + significant ( p< 0.05 ) according to Kruskall Wallis test +++ more highly significant ( p< 0.001 ) according to Kruskall Wallis test. * significant change ( p< 0.05 ) according to Mann-Whitney U test. *** more highly significant change ( p<0.001) according to Mann-Whitney U test.

  11. Table (5): Explain (Range ± Median) on the effect of apple juice contaminated with patulin (152.5 µg/1ml) on the aggressive behavior of albino mice. +++ more highly significant ( p< 0.001 ) according to Kruskall Wallis test. ** highly significant change ( p< 0.01 ) according to Mann-Whitney U test. *** more highly significant change ( p<0.001) according to Mann-Whitney U test

  12. Thank You

More Related