1 / 12

WP 5 – Fusarium and mycotoxin analysis

WP 5 – Fusarium and mycotoxin analysis. AVEQ -1st project meeting Clermont Ferrand, 19.-21.9. 2007. P4 Eurofins Analytik GmbH (Germany) - Scarlett Biselli P6 Institute for Cereal Research Bergamo (Italy) - Rita Redaelli

sanjiv
Download Presentation

WP 5 – Fusarium and mycotoxin analysis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. WP 5 – Fusarium and mycotoxin analysis AVEQ -1st project meeting Clermont Ferrand, 19.-21.9. 2007 P4 Eurofins Analytik GmbH (Germany) - Scarlett Biselli P6 Institute for Cereal Research Bergamo (Italy) - Rita Redaelli P8 Experimental Institute for Cereal Research of Fiorenzuola d´Arda (Italy) - Valeria Terzi P15 Agrotest Fyto, Ltd. (CR) - Ivana Polisenska

  2. WP 5 – Fusarium and mycotoxin analysis - what can we offer? AVEQ -1st project meeting Clermont Ferrand, 19.-21.9. 2007 P4 (Scarlett)- mycotoxin analyses LC-MS/MS (multitoxin method, DON, T-2, HT-2, zearalenone, diacetoxyscirpenol, 3- and 15- acetyldeoxynivalenol) - 200 samples P6, P8, P15 (Rita, Valeria, Ivana) - ELISA mycotoxin analyses, DON, T-2, ZEA - 2200 analyses P8 (Valeria) - Identification of Fusarium species - 200

  3. WP 5 – Fusarium and mycotoxin analysis - tasks and questions AVEQ -1st project meeting Clermont Ferrand, 19.-21.9. 2007 1) Which toxins are we to analyse? 2) Which Fusarium species are we to look for? 3) How to manage huge amount of samples? 4) Analyses; Methods

  4. 1) Which toxins are we to analyse? AVEQ -1st project meeting Clermont Ferrand, 19.-21.9. 2007 AVEQ = Avena genetic resources for quality in human consumption EU legislation setting maximum levels for certain contaminants in foodstuffs: Commission Regulation (EC) 1881/2006 DON (deoxynivalenol), ZEA, T-2 + HT-2 New discussed EU limits for T-2 and HT-2 (to be fixed in 200?)

  5. 1) Which toxins are we to analyse? AVEQ -1st project meeting Clermont Ferrand, 19.-21.9. 2007 DON; ZEA; T-2 + HT-2 cereals unprocessed 1250; 100; 100 ppb oats unprocessed 1750; 100; 500 ppb cereals intended for 750; 75; 200* ppb direct human consumption, cereal flour…*oat products The maximum level applies to unprocessed cereals placed on the market for first-stage processing. “First-stage processing” shall mean any physical or thermal treatment other than drying.

  6. 2) Which Fusarium species? AVEQ -1st project meeting Clermont Ferrand, 19.-21.9. 2007 What should be the purpose ... A) to analyse naturally infected oats to determine Fusarium species and their mutual proportions (variability in years, locations) B) to analyse inoculated oats - to confirm that (only) the Fusarium species which was used for inoculation is present ? - quantification of the amount of Fusarium mycelium for studying relationships between mycotoxins content ?

  7. 2) Which Fusarium species? AVEQ -1st project meeting Clermont Ferrand, 19.-21.9. 2007 A) to analyse naturally infected oats - which species? Tekauz and Mueller (2006) - in Canada F. graminearum, F. poae, F. sporotrichoides and F. avenaceum Parrika et al. (2006) - in Finland F. langsethiae, F. poae, F. culmorum, F. graminearum, F. avenaceum Imathiu et al. (2006) - in GB F. langsethiae CR - 2006, 9 oat samples, 16 Fusarium isolates 7x F. sporotrichoides 6x F. poae 2x F. culmorum ?F. langsethiae?

  8. 2) Which Fusarium species? AVEQ -1st project meeting Clermont Ferrand, 19.-21.9. 2007 F. langsethiae - newly described Fusarium species - similar to F. poae and F. sporotrichoides - produces T-2 and HT-2 toxins - isolated from all types of cereals, more common in oats - found in Sweden, Norway, Russia, Austria, Finland - hard artificial infection at flowering

  9. 3) Sample selection for analyses AVEQ -1st project meeting Clermont Ferrand, 19.-21.9. 2007 Inoculated plots: 300 accessions + 10 controls each year x 4 locations (Germany, Italy, Romania, CR) ______________ 1240 samples ELISA potential: 1100 analyses/per year (?number of mycotoxins analysed?) LC-MS/MS potential: 100 analyses/each year In case of 2 (DON, T-2): 550 samples ELISA + 100 HPLC/per year

  10. 3) Sample selection for analyses AVEQ -1st project meeting Clermont Ferrand, 19.-21.9. 2007 LC-MS/MS : - detection limit of 10 ppb - HT-2 toxin - more toxins in one analysis - expensive ELISA : - detection limit of 5-50 ppb - HT-2 toxin - one toxin in one analysis - reasonable costs Discussion about LC-MS/MS role - verification of ELISA results, interesting samples (low visible infection, low ELISA levels…) - analyses of other mycotoxins - comparative study - relationship between T-2 and HT-2 (naturally infected, inoculated)

  11. 4) Analyses, methods AVEQ -1st project meeting Clermont Ferrand, 19.-21.9. 2007 - harmonisation of the labs (1-2 reference samples, interlaboratory comparison) - the same kits for analyses ? - sample amount per analysis ? - logistic of sample distribution ?

  12. WP 5 - Time table

More Related