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Activity 11

Activity 11. Investigating Enzyme Function. What are some of the specific things that enzymes do in cells? What is unique about each protein’s structure that allows it to perform its function?. Read the introduction .

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Activity 11

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  1. Activity 11 Investigating Enzyme Function

  2. What are some of the specific things that enzymes do in cells? • What is unique about each protein’s structure that allows it to perform its function?

  3. Read the introduction. • An enzyme is a type of protein that speeds up, or catalyzes, a specific chemical reaction.

  4. Do you know anyone with lactose intolerance? • What do you know about the condition?

  5. lactase • Breakdown of lactose Lactose + water  glucose + galactose • Breakdown of glucose Glucose  carbon dioxide + water yeast

  6. Challenge • How do pH and temperature affect the function of the enzyme lactase?

  7. Safety Guidelines • Wear safety goggles at all times during this laboratory. • Keep your hands away from the hot plate, and wear insulated gloves or use potholders to move the beaker as you finish. • Know the safety procedures in case of fire. • Do not allow solutions to touch your skin or clothing. • Clean up spills immediately. • If accidental contact occurs, inform me, and rinse exposed areas.

  8. You must work within the following parameters: • The lactose should not be mixed with the lactase until after the lactase has been heated. • Once the yeast is added to a test tube, fix a balloon over it as quickly as possible before placing the tube in a water bath.

  9. Informal Meeting of the Minds • Find a partner from another group and compare the results of your investigations. • Go back to your group, share the new information with your partner, then share it with the other pair in the group.

  10. How Enzymes Work

  11. Analysis 4 • A. Does temperature affect the function of lactase? If yes, what is the effect? Explain, using evidence from the investigation. • B. Does pH affect the functioning of lactase? If yes, what is the effect? Explain, using evidence from the investigation.

  12. Analysis 6 • Most human cells function at a temperature of 37°C and a pH of around 7. From what you learned in this activity explain why it is important for humans to maintain temperature and pH homeostasis.

  13. Revisit the Challenge • How do pH and temperature affect the function of the enzyme lactase?

  14. How Enzymes Work

  15. Key Vocabulary • binding site • catalyze • enzyme • lactase • lactose • pH • protein

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