DNA to PROTEIN CHAPTER 12

1. DNA to PROTEINCHAPTER 12 • DEOXYRIBONUCLEIC ACID

2. DNA: replication and protein synthesis

3. Where have we seen DNA being replicated? MITOSIS AND MEIOSIS

4. Building blocks of DNA: Nucleotides

5. The sugar Deoxyribose The phosphate

6. The nitrogenous bases The Purines Why are these called nitrogenous bases?

7. The nitrogenous bases The Pyrimidines How are the pyrimidines different from the purines?

8. Four different Nucleotides BASIC STRUCTURE

9. DNA is a polymer formed by base pairing: Base pairing rule A pairs with T C pairs with G

10. The Double Helix • The overall shape of DNA is described as a double helix (a twisted ladder). • What force holds the two strands together?

11. How are DNA and RNA similar? • DNA is composed of nucleotides and RNA is composed of nucleotides

12. How are DNA and RNA different?

13. How are DNA and RNA different? • DNA… • Nucleotides = deoxyribose sugar • Double helix structure • Stays inside nucleus • RNA… • Nuleotides = ribose sugar • Single-strand structure • Located both inside and outside of nucleus • Uracil instead of thymine

14. DNA Replication • Set up your DNA by applying the base pair rules Strand 1 Complementary Strand 2 A T C G G

15. Enzymes involved in DNA replication • Helicase – opens the double helix to allow for replication • DNA polymerase – reads the original DNA strand and lays down complementary bases • Ligase – glues the newly formed DNA together

16. DNA replication practice • You are DNA polymerase. Helicase has opened the DNA strand – read each side and produce the complementary copies. __________________________________ A G G T A A C C G G T T A C G A T T A T T C C A T T G G C C A A T G C T A A T A A G G T A A C C G G T T A C G A T T A T T C C A T T G G C C A A T G C T A A T A

17. PARTNER PRACTICE • Person one uses their nucleotides as free nucleotides • Person two works with partner to replicate their original strand • Discuss the enzymes as you model the process

18. Do # 9 IN YOUR NOTES TO PRACTICE BASE PAIRING RULES AGAIN __________________________________ A G T C C G T T A G T T C A G G C A A T C A

19. Figure 12–7 Structure of DNA Section 12-1 Nucleotide Hydrogen bonds Sugar-phosphate backbone Key Adenine (A) Thymine (T) Cytosine (C) Guanine (G)

20. Use your text to complete the diagram and provide written details for the process shown

21. Homework • Complete labeling of notes cover • Complete DNA replication labeling and details • Complete Section 10-1 Review – accuracy!

22. Protein Synthesis= transcription and translation • DNA contains all the information for your traits – the genes • These genes are blueprints and need to remain safe – kept inside the nucleus • Copies can be made though – a messenger

23. Genotype  Phenotype DNA mRNA tRNA PROTEIN Transcription Translation (DNA to mRNA) (mRNA – tRNA to protein)

24. Messenger RNA Ribosomal RNA Transfer RNA Bringamino acids toribosome Combine with proteins tRNA mRNA Carry instructions rRNA DNA Ribosome Ribosomes Concept Map Section 12-3 RNA can be also called which functions to also called which functions to also called which functions to from to to make up

25. Transcription • #8 RNA polymerase reads one of the DNA strands and makes a complementary mRNA • #10 transcription details • Occurs in the nucleus • The gene sequence on DNA gets transcribed • Promoter region on DNA marks where transription should start and terminator region marks where it should stop

26. mRNA • RNA polymerase – key enzyme • mRNA is a “copy” of the gene sequence and can leave the nucleus • mRNA finds its way to a ribosme and the next step in making a protein can occur - TRANSLATION CLICK ON PICTURE FOR ANIMATION ON TRANSCRIPTION

27. mRNA • No T (thymine) so when it reads the nucleotide A on DNA it matches it with ____? • Do #11 in notes

28. #12 – TRANSLATION and tRNA • Once mRNA is made it attaches to a ribosome • tRNA = transfer RNA and they carry amino acids • Amino acids are the building blocks of proteins (remember?)

29. Translation • Ribosomes are the site of protein synthesis • Click here to see mRNA and tRNA work together at that ribosome to build a protein

30. Codon = mRNAAnti-codon = tRNA

33. Copy down this DNA sequence CAG GTG AAT TGG GGC CAC CAC TTT • This is the template strand of DNA, complete the complementary strand sequence below the template. • TRANSCRIPTION: read the template DNA strand and write the complementary mRNA • TRANSLATION: based on your mRNA, determine the proper amino acid sequence

34. Copy this DNA sequence down CAG GTG AAT TGG GGC CTC CAC TTT REPEAT ALL THE STEPS AND DETERMINE THE AMINO ACID SEQUENCE FOR THIS GENE!

35. COMPARE: what is the mistake? • CAG GTG AAT TGG GGC CAC CAC TTT • CAG GTG AAT TGG GGC CTC CAC TTT

36. One incorrect amino acid

37. GENOTYPE to PHENOTYPE

38. Figure 12–20 Chromosomal Mutations Section 12-4 Deletion Duplication Inversion Translocation

39. Let’s Review • DNA Structure is a _____ ______ • DNA is composed of __________What are four that make up DNA? • A • T • C • G

40. Figure 12–5 DNA Nucleotides Section 12-1 Purines Pyrimidines Adenine Guanine Cytosine Thymine Phosphate group Deoxyribose

41. Figure 12–7 Structure of DNA Section 12-1 Nucleotide Hydrogen bonds Sugar-phosphate backbone Key Adenine (A) Thymine (T) Cytosine (C) Guanine (G)

42. Use your text to complete the diagram and provide written details for the process shown

43. Messenger RNA Ribosomal RNA Transfer RNA Bringamino acids toribosome Combine with proteins tRNA mRNA Carry instructions rRNA DNA Ribosome Ribosomes Concept Map Section 12-3 RNA can be also called which functions to also called which functions to also called which functions to from to to make up

44. Figure 12–14 Transcription Section 12-3 Adenine (DNA and RNA) Cystosine (DNA and RNA) Guanine(DNA and RNA) Thymine (DNA only) Uracil (RNA only) RNApolymerase DNA RNA

45. Quest Answers 8 A 9 C 10 A 11C 12 B 13B 1 C 2 AB 3 E 4 B 5 AD 6 AC 7 D