General Microbiology

1. General Microbiology Nickolas V. Kapp Ph.D

2. Office: 738-4415 E-mail: Kapp@smccd.net FAX: 738-4499 Office 7224 Office hours:M,W,F 9 to 11. TTh By Arrangement How to get a hold of Nick

3. The micro class • We will normally have lecture from 9:35 till 10:50. • See Course Outline • Attendance and promptness will count towards your grade

4. What if I want to look at my plates at some other times? • Open Lab hours are • Mon and Wed 9 to 12 • Friday 10-2 • Check the notice on the lab door • Remember you are working with live organisms and they have their own time schedule. Someone from your lab group will have to check on your materials

5. Looking at plates during other classes • Mostly no • If you must make some observations during another lab class • Don’t bother a lecture in progress • Find the instructor and ask • Be prepared for a no • You are meeting a possible instructor for your next class

6. Materials required for this class. • Text, Totora, Funke and Case Microbiology: An Introduction, 10th ed. • Case and Johnson Laboratory Experiments in Microbiology 9th ed. • A lab coat or a large Lab shirt to cover yourself. • Safety Glasses • NO eating in the laboratory

7. As you can see Sometimes there is a blur between what we do in lab and what we do in class.

8. Evaluation • See class outline

9. A 90% and above B 80%90% C 68%-80% D 50%-67% Fail below 50% Attendance will be taken in the first minutes of class. Each absence will result in the loss of points from the total possible. Grading Scale

10. Extra Credit is possible.

11. Participation Credit • Joining ASM or NCMS (5pt) • Answer question or ask one 1pt • Enter microbe of the month 1pt • Attend a meeting or lecture on microbiology and hand in a report (10pt) • Field trip (to be announced) (5pt) • Max of 15pt

12. While some of the lecture material will change The Exam dates will not.

13. Nick Kapp Ph.D. 7384415 Kapp@smccd.net 8224

14. What is a Microbe • Smaller than 0.1mm • Includes bugs, things, germs, viruses, protozoan, bacteria, animalcules, small suckers

15. Nomenclature • Carolus Linnaeus (1735) • Genus species • By custom once mentioned can be abbreviated with initial of genus followed by specific epithet. E. coli • When two organisms share a common genus are related.

16. Why study Microbiology • Microbes are related to all life. • In all environments • Many beneficial aspects • Related to life processes (food web, nutrient cycling) • Only a minority are pathogenic. • Most of our problems are caused by microbes

17. EID’s • Emerging infectious diseases • Weapons of mass destruction • New evolutionary features • Response to man encroaching on the environment • Can you name an example?

18. Microbes in research • 10 trillion human cells 10x this number microbes • Easy to grow • Biochemistry is essentially the same • Simple and easy to study

19. Biotechnology • Use of biological systems to produce useful items • The use of biological information to make things or improve the human condition

20. Diversity of Microbes • Bacteria-single celled prokaryotes • Protozoa-eukaryotic, single celled, colonial, many ways of nutrition • Fungi- absorb nutrients, single celled filamentous • Viruses-acellular entities • Others- worms, insects

22. Bacteria • Prokaryotes • Peptidoglycan cell walls • Binary fission • For energy, use organic chemicals, inorganic chemicals, or photosynthesis Figure 1.1a

23. Archaea: • Prokaryotic • Lack peptidoglycan • Live in extreme environments • Include: • Methanogens • Extreme halophiles • Extreme thermophiles Halobacteria not from book

24. Fungi • Eukaryotes • Chitin cell walls • Use organic chemicals for energy • Molds and mushrooms are multicellular consisting of masses of mycelia, which are composed of filaments called hyphae • Yeasts are unicellular Figure 1.1b

25. Protozoa • Eukaryotes • Absorb or ingest organic chemicals • May be motile via pseudopods, cilia, or flagella • Most free some parasites Figure 1.1c

26. Algae • Eukaryotes • Cellulose cell walls • Use photosynthesis for energy (primary producers) • Produce molecular oxygen and organic compounds • Metabolically diverse Figure 1.1d

27. Viruses • Acellular • Consist of DNA or RNA core • Core is surrounded by a protein coat • Coat may be enclosed in a lipid envelope • Viruses are replicated only when they are in a living host cell Figure 1.1e

28. Multicellular Animal Parasites • Eukaryote • Multicellular animals • Parasitic flatworms and round worms are called helminths. • Microscopic stages in life cycles. Figure fluke

29. The Scientific Method • Make an observation • Make a hypothesis • Test the hypothesis • Draw your conclusions • repeat

30. Requirements for Scientific methods • Single variables • Experimental controls • How can this be used to discover things? • Does HIV cause AIDS??? Discuss

31. Knowledge of microorganisms: • Allows humans to • Prevent food spoilage • Prevent disease occurrence • Others? • Led to aseptic techniques to prevent contamination in medicine and in microbiology laboratories.

32. Universal precautions set up by CDC • Use gloves, gowns, masks and goggles • Minimize risk of needle sticks • Disinfections procedure • Preventative treatment after exposure • Reduce risk • Treat all patients the same • HBV greater risk than HIV

33. The Debate Over Spontaneous Generation • The hypothesis that living organisms arise from nonliving matter is called spontaneous generation. According to spontaneous generation, a “vital force’ forms life. • The Alternative hypothesis, that the living organisms arise from preexisting life, is called biogenesis.

34. Evidence Pro and Con • 1668: Francisco Redi filled six jars with decaying meat.

35. Evidence Pro and Con • 1765: Lazzaro Spallanzani boiled nutrient solutions in flasks.

36. The Theory of Biogenesis • Pasteur’s S-shaped flask kept microbes out but let air in. Figure 1.3

37. Where is Microbiology currently being practiced? I.e. jobs • Put your Choice here

38. A timeline of Microbiology • Fig 1.4 • Some highlights • 1665 Hooke • 1673 van Leeuwenhoek’s microscopes • 1735 Linnaeus Nomenclature • 1798 Jenner vaccine • 1857 Pasteur Fermentation • 1876 Koch germ theory of disease

39. The Golden Age of Microbiology • 1857-1914 • Beginning with Pasteur’s work, discoveries included the relationship between microbes and disease, immunity, and antimicrobial drugs

40. Fermentation and Pasteurization • Pasteur showed that microbes are responsible for fermentation. • Fermentation is the conversation of sugar to alcohol to make beer and wine. • Microbial growth is also responsible for spoilage of food. • Bacteria that use alcohol and produce acetic acid spoil wine by turning it to vinegar (acetic acid).

41. Fermentation and Pasteurization • Pasteur demonstrated that these spoilage bacteria could be killed by heat that was not hot enough to evaporate the alcohol in wine. This application of a high heat for a short time is called pasteurization. Figure 1.4

42. The Germ Theory of Disease • 1835: Agostino Bassi showed a silkworm disease was caused by a fungus. • 1865: Pasteur believed that another silkworm disease was caused by a protozoan. • 1840s: Ignaz Semmelwise advocated hand washing to prevent transmission of puerperal fever from one OB patient to another.

43. The Germ Theory of Disease • 1860s: Joseph Lister used a chemical disinfectant to prevent surgical wound infections after looking at Pasteur’s work showing microbes are in the air, can spoil food, and cause animal diseases. • 1876: Robert Koch provided proof that a bacterium causes anthrax and provided the experimental steps, Koch’s postulates, used to prove that a specific microbe causes a specific disease.

44. The Birth of Modern Chemotherapy • Treatment with chemicals is chemotherapy. • Chemotherapeutic agents used to treat infectious disease can be synthetic drugs or antibiotics. • Antibiotics are chemicals produced by bacteria and fungi that inhibit or kill other microbes. • Quinine from tree bark was long used to treat malaria. • 1910: Paul Ehrlich developed a synthetic arsenic drug, salvarsan, to treat syphilis. • 1930s: Sulfonamides were synthesized.

45. The Birth of Modern Chemotherapy • 1928: Alexander Fleming discovered the first antibiotic. • He observed that Penicillium fungus made an antibiotic, penicillin, that killed S. aureus. • 1940s: Penicillin was tested clinically and mass produced. Similar to Figure 1.5

46. Modern Developments in Microbiology • Bacteriology is the study of bacteria. • Mycology is the study of fungi. • Parasitology is the study of protozoa and parasitic worms. • Recent advances in genomics, the study of an organism’s genes, have provided new tools for classifying microorganisms. • Proteomics is looking at the gene products

47. Selected Novel Prizes in Physiology or Medicine • 1901* von Behring Diphtheria antitoxin • 1902 Ross Malaria transmission • 1905 Koch TB bacterium • 1908 Metchnikoff Phagocytes • 1945 Fleming, Chain, Florey Penicillin • 1952 Waksman Streptomycin • 1969 Delbrück, Hershey, Luria Viral replication • 1987 Tonegawa Antibody genetics • Prusiner Prions • Agre, Mackirron water and ion channels • 2005 Marshall, Warren Helicobacter and ulcers • 2008 Hausen Papilloma and viruses * The first Nobel Prize in Physiology or Medicine.

48. Principles of Microscopy • Metric units (table 3.1) • Micrometer • Nanometer • angstrom

49. Compound light microscopy • Basic parts • Eyepieces (ocular lens) • Base • Condenser • Iris diaphragm • Objective lens • Body tube • Mechanical stage • Adjustment knobs

50. Magnification • Calculation: • Objective power x ocular power = total power • Parafocial • Paracentric • Microscopic measurement • Micrometer? Why must we calibrate it?