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10 mL of TSB

Media Fill Procedure. 3 mL Shielded syringe. 10 mL of TSB. 1 mL. 20 or 30 mL shielded evac vial. (simulatedTc-99m). X 4. 10 mL unshielded syringe. (repeat 3 more times for a total of 4 vials). Simulated Kit vial #1. Source Vial of TSB. 3 mL. Draw 0.4 mL of

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10 mL of TSB

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  1. Media Fill Procedure 3 mL Shielded syringe 10 mL of TSB 1 mL 20 or 30 mL shielded evac vial (simulatedTc-99m) X 4 10 mL unshielded syringe (repeat 3 more times for a total of 4 vials) Simulated Kit vial #1 Source Vial of TSB 3 mL Draw 0.4 mL of media from Kit (simulated normal saline)

  2. Media Fill Procedure (Cont’d) Kit # 1 Kit #2 Kit #3 Kit #4 Draw 0.4 mL of media from Kit 3 mL shielded syringes Repeat 3 times for a total of 4 vials and 24 syringes 10 or 20 mL incubation vials

  3. 1 2 3 4 Media Fill Procedure (Cont’d) • Incubate 4 new vials at 25-35 Degrees Celsius in a controlled environment for 14 days.

  4. 1 Media Fill Procedure (Overview) 5 mL Shielded syringe 10 mL of TSB 1 mL 20 or 30 mL shielded evac vial Tc-99m 10 mL unshielded syringe X 4 Vial of TSB (repeat 4 times) Simulated Kit vial #1 3 mL Draw 0.4 mL of media from Kit 3 or 5 mL shielded syringes Repeat process 4 times for a total of 24 incubation vials 10 or 20 mL incubation vials Incubate vials at 25-35 Degrees Celsius

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