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Experience with SARS Laboratory Diagnostics

Experience with SARS Laboratory Diagnostics. JSM Peiris, LLM Poon, Y Guan, KH Chan. The University of Hong Kong & Queen Mary Hospital. Early Response: March 28. I st Generation assays RT-PCR for P gene Indirect immuno-fluorescence serology using virus infected cells. 0. 10,000.

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Experience with SARS Laboratory Diagnostics

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  1. Experience with SARS Laboratory Diagnostics JSM Peiris, LLM Poon, Y Guan, KH Chan. The University of Hong Kong & Queen Mary Hospital

  2. Early Response: March 28 Ist Generation assays • RT-PCR for P gene • Indirect immuno-fluorescence serology using virus infected cells

  3. 0 10,000 20,000 29,736 nt Orf1ab polyprotein Orf1a polyprotein E2 glycoprotein E1 protein M protein N protein positive negative H20 RT-PCR tests: 1st Generation

  4. 2nd Generation RT-PCR testsApril 03 Real Time RT-PCR Roche Light Cycler

  5. Viral load in Nasopharyngeal aspirates Peiris et al 2003, Lancet 361: 1767-72

  6. ABI GeneAmp 7000 using TaqMan detection • Run capacity 96 specimens • Run time 1.5 hrs • Specificty- Probe • Compare different gene targets • P gene • N gene

  7. water 10e4 10e5 10e1 10e6 10e2 10e3 R2:0.99 TaqMan assay for polymerase gene 10e6 10e5 10e4 10e3 10e2 10e1

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