1 / 34

In the name of GOD

In the name of GOD. Molecular imprinted polymer (MIP). Supervisor Dr. Parchehbaf Presenter Meysam abdi 1391/09/15 2012/12/05. Content. Introduction History Principles Advantage Application Conclusion. Introduction. Base of the method, is recording of target molecule structure

sondra
Download Presentation

In the name of GOD

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. In the name of GOD

  2. Molecular imprinted polymer(MIP) Supervisor Dr. Parchehbaf Presenter Meysamabdi 1391/09/15 2012/12/05

  3. Content • Introduction • History • Principles • Advantage • Application • Conclusion

  4. Introduction Base of the method, is recording of target molecule structure In Polymer memory at polymerization process. so that the identification, synthesized polymer only detects the target molecule.

  5. History • 1894, Fischer lock & key theory. • 1930, Polyakov, molecular imprinting in silica’s matrix • 1949, Linuspauling theory, change of antibody structures • 1977, Gunter wulff, molecular imprinting by using covalence banding • 1981, Klaus mosbach, molecular imprinting by using non-covalence banding • 1995, Witcombe, molecular imprinting by using semi-covalence (hybrid) banding www.wikipedia.com www.sciencedirect.com

  6. Research procedure C. Alexander, L. Davidson, and W. Hayes, Tetrahedron 59, 2025-2057 (2003).

  7. Principles www.biotage.com

  8. Fischer Lock & Key theory bonding Remove template molecule PowerPoint of J.jafari thesis Monomer & cross linker Template molecule

  9. Variety methods of MIP production

  10. Advantage • high selectivity • high pre-Concentration factor • the sorbent is reusable for many times

  11. Application of MIP

  12. Sensor Development of a voltammetric sensor based on a molecularly imprinted polymer(MIP) for caffeine measurement Step1:A caffeine-selective molecularly imprinted polymer (MIP) and a non-imprinted polymer (NIP) synthesized. Step2: The MIP, embedded in the carbon paste electrode. Step3:The prepared electrode was used for caffeine measurement via: 1.analyte extraction in the electrode 2.electrode washing 3.electrochemical measurement of caffeine. Step4: DPV were used for determination. A linear range of calibration curve: 6×10-8 to 2.5×10-5 mol/L The detection limit: 1.5×10-8 mol/L. T. Alizadeh et al. / Electrochimica Acta 55 (2010) 1568–1574

  13. Caffeine (1,3,7-trimethylxanthine) is an alkaloid

  14. MIP-CP & NIP-CP responses

  15. MIP-CP electrode selectivity

  16. Real sample analysis

  17. A novel high selective and sensitive para-nitrophenol volta- -mmetric sensor, based on a MIP-CP electrode Step1: Para-nitrophenol selective MIP and a non-imprinted polymer (NIP) synthesized Step2:used for carbon paste (CP) electrode preparation. Step3:A dynamic linear range of 8×10-9 to 5×10-6 mol /L was obtained. The detection limit of the sensor was calculated as 3×10-9 mol/L. T. Alizadeh et al. / Talanta 79 (2009) 1197–1203

  18. Electrode responses & washing effect

  19. Selection of elecrochemical methods

  20. Analytical characterization

  21. Application of an Hg2+ selective imprinted polymer for the preparation of a novel highly selective and sensitive electrochemical sensor for the determination of ultra trace Hg2+ Step1: Mercury ion selective cavities were created in the vinyl pyridine based cross-linked polymer. Step2: The interference of different ionic species with the response of the electrode was also studied. Step3: This sensor showed a linear response range of 2.5×10-9 to 5.0×10-7 M and lower detection limit of 5.2×10−10 M (S/N). T. Alizadeh et al. / AnalyticaChimicaActa689 (2011) 52–59

  22. Comparison of the IIP-CP & NIP-CP electrodes

  23. Evaluation the selectivity of sensor

  24. Determination of Hg2+ in real samples

  25. Preparation of nano-sized Pb2+ imprinted polymer and its application as the chemical interface of an electrochemical sensor for toxic lead determination indifferent real samples Step1: a new nano-structured ion imprinted polymer (IIP) was synthesized by copolymerization Step2: A carbon paste electrode modified with IIP-nanoparticles was used for fabrication of a Pb2+ sensitive electrode. Step3: Differential pulse stripping voltammetry method was applied as the determination technique, Step4: The introduced sensor showed a linear range of 1.0×10-9 to 8.1×10-7 M and detection limit of 6.0×10-10 (S/N = 3). T. Alizadeh, S. Amjadi / Journal of Hazardous Materials 190 (2011) 451–459

  26. Comparison of the prepared electrodes

  27. Evaluation of the selectivity of IIP-CP electrode

  28. Evaluation of the effect of extraction conditions

  29. Determination of Pb2+ in real samples by proposed sensor T. Alizadeh et al. / Talanta 79 (2009) 1197–1203

  30. Prepared electrode T. Alizadeh et al. / Talanta 79 (2009) 1197–1203

  31. Conclusion a new electrochemical sensor for determination of compounds at trace levels was introduced. Application of polymer as a novel modifying agent in the carbon paste electrode made it very selective for compounds determination in the presence of common potential interfering agents. The polymer, used in the carbon paste composition, acted as the selective chemical interface of the sensor as well as a pre-concentration agent.

  32. Thanks for your attention template of PowerPoint from: www.m62.net

More Related