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Pathology with Paper and Pencil

Develop a glucose detection process and blood cell separation using a paper microchannel filter paper. Fabricate complex structures for protein assay and antimicrobial susceptibility tests on the paper-based kits. Explore multiplexing capabilities for simultaneous analyte detection like glucose and protein, and blood group identification. Calibrate the device for accurate colorimetric blood glucose detection. Collaboration with researchers and students to advance the prototype towards commercialization.

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Pathology with Paper and Pencil

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  1. Pathology with Paper and Pencil [Ref: P. Mandal, R. Dey, S. Chakraborty, Lab Chip, 2012, 12, 4026-4028] Normal filter paper Filter paper soaked in photoresist; prebaked Mask for UV exposure Paper channel

  2. Fabrication of Complex Structures on Paper Zigzag design to investigate mixing H-channel designed to investigate blood cell separation 13 11 9 7 5 3 1 12 10 8 6 4 2

  3. Colorimetric Detection of Blood Glucose and Multiplexing Glucose detection process Calibration with standard glucose samples Brown color developed Paper microchannel Glucose Reagent Blood Sample Separation + Reaction Reagents • Whatman grade 1 • Length = 20 mm • W = 3 mm, Φ = 7 mm Plasma separated from RBC Blood sample to source pad Antibody (ABO) Calibration curve with Standard glucose Image Acquisition Image Processing Result Display Protein assay Anti A-B Multiplexing: • Simultaneous detection of multiple analytes like glucose and protein etc. • Blood group Identification Anti O Glucose assay

  4. Paper-based Kits for Antimicrobial Susceptibility Assays Prototype: Indian patent file no: 201631023867 Working protocol: Microfluidics and Microscale Transport Processes Laboratory

  5. Towards a Commercializable Device Functional block diagram of the med Colorimeter. Paper Based Micro-fluidic Sensor with Blood Sample Micro-controller/ Digital Signal Processor LED based calibrated light source Calibrated photo detector Trans-impedance amplifier + filters Analog to Digital Converter Smart Phone Bluetooth Low Energy Transceiver A/Prof. Sudip Nag Prof. Suman Chakraborty

  6. Prototype Design Paper Based Blood Sample Sensor Battery Calibrated LED with Driver and Optical Detector Opaque Enclosure Bluetooth Low Energy Transceiver

  7. Specification of the Prototype

  8. Acknowledgement Faculty Member Dr. Sudip Nag Department of E&ECE IIT Kharagpur, INDIA Researchers and Students Dr. Shantimoy Kar (former Ph.D student) SujayBiswas (current Ph.D student)

  9. Acknowledgement Researchers and Students Dr. Shantimoy Kar SujayBiswas

  10. Collaborative partners and funding Agencies

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