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2. DNA micelles prepared with randomly varying PEG-poly(kSer) and PEG- poly(Lys) polypeptides

Optimized Rratios of Acid-Degradability/Complexation Stability for Nonviral Gene Delivery via Acid-Transforming Polypeptide Micelles Young Jik Kwon, University of California-Irvine, DMR 0956091.

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2. DNA micelles prepared with randomly varying PEG-poly(kSer) and PEG- poly(Lys) polypeptides

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  1. Optimized Rratios of Acid-Degradability/Complexation Stability for Nonviral Gene Delivery via Acid-Transforming Polypeptide Micelles Young Jik Kwon, University of California-Irvine, DMR 0956091 Goal of the study: To obtain the optimized formulation of acid-transforming polypeptides (ATPPs) for nonviral gene delivery (kSer: acid-degradable ketalized serine; Lys: lysine; PEG: polyethylene glycol). Approach: To assess transfection efficiency by 1) PEG-poly[kSer-(Lys)n]/DNA micelles (i.e., defined polymeric structure) and 2) polyplexes prepared with PEG-poly(kSer) and PEG-poly(Lys) (i.e., randomly varied acid-degradability vs. micelle structure). Findings: 1) Unexpectedly, full acid-degradability (contributed by kSer) of nonviral gene carriers did not result in the most efficient transfection and 2) addition of non-degradable Lys residues enhanced transfection efficiency. Conclusion: Ideal nonviral gene delivery carriers should have a optimally balanced stimuli-responsive degradability as well as complexation stability at a certain level. 1. PEG-poly[kSer-(Lys)n]/DNA micelles 2. DNA micelles prepared with randomly varying PEG-poly(kSer) and PEG- poly(Lys) polypeptides

  2. Summer Outreach Research in Gene Delivery at UC Irvine for Under-Represented High School StudentsYoung Jik Kwon, University of California-Irvine, DMR 0956091 Nine students (4 female; 5 Hispanic; 1 economically disadvantaged) from four high schools in Orange County spent a week in the PI’s laboratory (July 26-30, 2010). They conducted a demonstrative project on transfecting mouse fibroblast cells using GFP gene-carrying polymeric nanoparticles, after completing required safety training. They learned cell culture, gel electrophoresis, dynamic light scattering particle size measurement, TEM, fluorescence microscopy, and flow cytometry. On the presentations to their families and the PI on the last day of the program, they commented, “ I learned that research could possibly the path I want to take for the rest of my life”, “It was life-changing experience”, and “I can’t thank Dr. Kwon and NSF enough for this amazing program. It was truly awesome”.

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