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PSEUDOMONAS spp.

PSEUDOMONAS spp. Lecture 12. Prepared by: Miss Norzawani Jaffar , Bsc ( Hons ) Biomedical Sciences, UKM. Learning Outcomes. At the end of learning session, student must be able to: Describe characteristic of Pseudomonas sp. Explain the morphology and classification of Pseudomonas sp.

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PSEUDOMONAS spp.

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  1. PSEUDOMONAS spp. Lecture 12 Prepared by: Miss NorzawaniJaffar, Bsc (Hons) Biomedical Sciences, UKM

  2. Learning Outcomes At the end of learning session, student must be able to: • Describe characteristic of Pseudomonas sp. • Explain the morphology and classification of Pseudomonas sp. • Describe the laboratory test that significant for Pseudomonas sp identification. • Explain the related disease and infections that potentially cause by Pseudomonas sp.

  3. Pseudomonas sp Scientific classification • Domain: Bacteria • Phylum: Proteobacteria • Class: Gammaproteobacteria • Order: Pseudomonadales • Family: Pseudomonadaceae • Genus: Pseudomonas

  4. Pseudomonas sp • Strict aerobes widely distributed in nature • They are mainly opportunist pathogens infecting those with impaired septicaemia. • Some rare GNRs are environmental organism causing hospital-acquired infections from contaminated fluids or equipment. • Other rare Gram negative rods are normal or animal flora causing opportunistic infections including dental infections, infected bites and systemic infections. • Pseudomonal species have been found in soil, water, plants, and animals; Pseudomonas aeruginosa colonization reportedly occurs in more than 50% of humans, and P aeruginosa is the most common pseudomonal species.

  5. Characteristic Members of the genus display the following defining characteristics; • Rod shaped • Gram-negative • One or more polar flagella, providing motility • Aerobic • Non–spore forming • positive catalase test • positive oxidase test. • Beta hemolytic (on blood agar) • Indole negative • Methyl red negative • Citrate positive.

  6. Pseudomonas colonies on agar plate

  7. Pseudomonas with flagella

  8. Diseases

  9. Pseudomonal infection, as described by Pollack, occurs in 3 stages; • (1) bacterial attachment and colonization, followed by • (2) local invasion • (3) dissemination and systemic disease

  10. End…….. Q & A

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