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BIOSORPTION OF COBALT AND COPPER FROM HYDROMETALLURGICAL SOLUTIONS MEDIATED BY Pseudomonas spp

BIOSORPTION OF COBALT AND COPPER FROM HYDROMETALLURGICAL SOLUTIONS MEDIATED BY Pseudomonas spp. PRESENTATION BY: NONJABULO PRUDENCE DLAMINI. PRESENTATION LAYOUT. Introduction Scope of the work Experimental design Results & discussions Conclusions. INTRODUCTION.

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BIOSORPTION OF COBALT AND COPPER FROM HYDROMETALLURGICAL SOLUTIONS MEDIATED BY Pseudomonas spp

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  1. BIOSORPTION OF COBALT AND COPPER FROM HYDROMETALLURGICAL SOLUTIONS MEDIATED BY Pseudomonas spp PRESENTATION BY: NONJABULO PRUDENCE DLAMINI

  2. PRESENTATION LAYOUT • Introduction • Scope of the work • Experimental design • Results & discussions • Conclusions

  3. INTRODUCTION • The use of micro-organisms in the dissolution of metals bearing minerals and the removal of some anions e.g. phosphates and ammonia is well known e.g. sulphur oxidisers Acidithiobacillus spp, Leptospirillum spp and Sulpholobus spp. (Acharya,1990) (Momba, 2005). • However, not much has been done on the use of microorganisms in the removal of leached metals from their aqueous solutions. E.g. Fixation of Ni and Fe by Pseudonomonas species throughbiosorption (Danalkova et al,2005). • In this study copper and cobalt are being removed from hydrometallurgical solutions using microorganisms

  4. The use of biological methods for heavy metal recovery have gained considerable momentum due to their high efficiency, low operating costs and simplicity.

  5. SCOPE OF THE WORK • This project was aimed at testing the viability of using bacteria as; • Alternatives to costly metal removal techniques (water purification). • Biosorbents to recover precious metals from hydrometallurgical process solutions.

  6. EXPERIMENTAL DESIGN • Sampling • Soil & water samples were collected from water bodies around the small scale mines.

  7. Sampled sites • Acidic • Turbidity high • Conductivity high

  8. CULTURING AND IDENTIFICATION • Soil samples were suspended in distilled water, vortexed (thoroughly mixed). • Both the water and soil samples supernatant were diluted in a sterile phosphate buffer and there after inoculated in Pseudomonas agar base. • The isolation of bacteria using Pseudomonas agar base yielded positive results meaning that there blue-green colonies of Pseudomonas spp were observed.

  9. Synthetic solutions • Solutions of different concentrations (0.07M, 0.33M and 0.66M) were prepared using copper and cobalt sulphate. • Batch scale experiments to test the effects of • Contact time, concentration, pH, temperature, co-ions, biomass concentration were conducted.

  10. Metal recovery • Bacteria : water (3:1) ratio • Bacteria were inoculated into synthetic solutions water samples and recovery was allowed to take place over a period of 24 h in a shaking incubator (150 rpm) at 36.5°C. • Metal concentrations were analysed using AAS and ICP. • Control experiments were conducted to monitor parameters like precipitation. • The same procedure was repeated for hydrometallurgical solutions which had lower metal concentrations.

  11. Results & discussions • Contact time and temperature had an almost similar effect on the rate of biosorption. Increased up to a certain level then decreased.

  12. pH • Maximum uptake of Cu and Co by Pseudomonas spp was attained at pH 6.5.

  13. Biomass (bacterial concentration) 3 : 1

  14. Co-ions • Mixing copper & cobalt enhanced removal of copper. • Ionic radii

  15. Kinetic models Langmuir equation model R2= 0.8615

  16. Freundlich equation model The Freundlich equation gave a plot with higher correlation as compared to the Langmuir equation. The Freundlich gave an R2 value of 0.9622 whereas the Langmuir had an R2 value of 0.8615. Therefore the Fruendlich plot is favoured over the Langmuir. R2=0.9622

  17. Recovery from hydrometallurgical solutions

  18. INTERACTION OF MICROBES WITH METALS (SEM) SEM micrograph of the dried metal solution before interaction with Pseudomonasspp rods. SEM micrograph showing the dried mixture of metal solution with Pseudomonas spp

  19. TEM metal bioaccumulation on the periplasmic wall of the Pseudomonas spp rods.

  20. Conclusions • Pseudomonas spp reclaim both copper and cobalt from their sulphate synthetic solutions . • It tends to remove or extract more metal ions at low concentrations and the ratio of bacteria population to solution volume being 3:1 works best. • The maximum uptake of both copper and cobalt occurred at a pH of 6.5 beyond which the adsorption rates decreased. • The bacteria have successfully removed up to 45 % of copper from 0.07M and up 40 % from 0.07M cobalt from cobalt sulphate solutions and 73 % and 65 % of copper and cobalt respectively from mine water samples.

  21. ACKNOWLEDGEMENTS • Nigel town municipality and small scale miners. • National research foundation and the University of Johannesburg for financial assistance. • Mr E. Fosso-Kankeu

  22. ….THANK YOU… …….THANK YOU…… ?????

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