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HisH - HisF

HisH - HisF. Imidazole Glycerol Phosphate Synthase: regulates 5 th step histidine biosynthesis HisH class I glutamine amidotransferase HisF alpha-beta barrel fold, cyclase rxn Recently suggested hisF uses barrel as efficient intermediate channel Ammonia conduction, gating mechanism

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HisH - HisF

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  1. HisH - HisF • Imidazole Glycerol Phosphate Synthase: regulates 5th step histidine biosynthesis • HisH class I glutamine amidotransferase • HisF alpha-beta barrel fold, cyclase rxn • Recently suggested hisF uses barrel as efficient intermediate channel • Ammonia conduction, gating mechanism • Modeling activated complex requires parameterization P. O’Donoughue, R. Amaro, Z. Schulten, J Struct Biol, 2001.

  2. HisH

  3. HisH HisH active site: Catalytic triad CYS84 – HIS178 – GLU180

  4. HisF

  5. HisF Active site residues

  6. Top View of HisF

  7. Conserved gate residues Form stable salt bridges Gate diameter ~ 3 Å

  8. Predominantly hydrophobic channel

  9. Docked Complex Crystal Structure Douangamath et al., Structure, Feb. 2002. PDB code: 1GPW

  10. Hypothetical Coupling Mechanism Glutamine binds in hisH active site

  11. Hypothetical Coupling Mechanism Glutamine binds in hisH active site PRFAR binds to hisF active site

  12. Hypothetical Coupling Mechanism Glutamine binds in hisH active site PRFAR binds to hisF active site Activated event

  13. Hypothetical Coupling Mechanism NH3 released in 5th reaction

  14. Hypothetical Coupling Mechanism NH3 diffuses across interface

  15. Hypothetical Coupling Mechanism NH3 diffuses across interface

  16. Hypothetical Coupling Mechanism NH3 travels ~10Å to mouth of hisF

  17. Hypothetical Coupling Mechanism NH3 travels ~10Å to mouth of hisF NH3 passes through channel ~15Å To participate in ImGP formation

  18. What is known experimentally • Crystal structures of both bacterial and eukaryotic1 organisms (2001) • Mutational studies involving residues of both subunits in gate and at interface2 • ARG5 and GLU46 play essential roles in rxn • The activity of hisH is dependent on the binding of the substrate at the hisF active site 1Chaudhuri et al., Structure, 2001. 2Klem et al., J Bactero., 2001; Beismann-Driemeyer, J Biol Chem, 2001

  19. Novel function for ubiquitous fold • Substrate channeling common in glutamine amidotransferases since coupled to second reaction requiring reactive ammonia • Allows protected / directed travel of intermediate • Coupling two sequential reactions increases enzymatic regulation • First time α/β barrel proposed to be used as an intermediate channel !

  20. Investigating the Gate Mechanism • Gate seems closed in crystal structures • Diameter of gate 3.2Å, NH3 is ~ 2Å • Use bioinformatics to narrow the search • 2 conserved ASP’s near gate • Salt bridges could be formed between ASP98 – LYS99 and/or ASP219—ARG5 • Increases diameter of gate to 6.9Å • Stable! Stay in formation for ps

  21. Gate at entrance of hisF barrel Crystal structures all in closed gate conformation

  22. Simulated Gating Mechanisms • Followed suggestion by Chaudhuri et al. to form a hydrogen bond between 2 strictly conserved residues at the interface: TYR138 of hisH and LYS99 of hisF’s gate • Increased the diameter of the channel from 3.2Å to 5.8Å • Since no experimental evidence for any gating mechanism, also simulated the closed gate

  23. Strictly conserved TYR 138 of hisH Possible gating mechanism?

  24. System Setup Started with 2.4Å resolution crystal structure

  25. System Setup Started with 2.4Å resolution crystal structure Solvated complex with explicit waters Minimized, equilibrated using NAMD2 and Charmm27 force field in NPT ensemble Theoretical Biophysics Group, K. Schulten et al.

  26. Ammonia Conduction • SMD to induce the passage of ammonia through the channel on the ns timescale • Hamiltonian of the system becomes: • NH3 through the channel at constant v = 15Å/ns • Analyzed the resulting trajectories, forces

  27. Energy Barrier of Gating Mechanism closed gate entrance ~25-40 kcal/mol open gate entrance ~3 kcal/mol

  28. Free Energy Profile in Channel THR78 SER101 SER201

  29. A more realistic model • Would like to model “activated” complex • Needed to build in both substrates • hisH: covalently bound glutamine / glutamate • hisF: substrate PRFAR • No publicly available parameters for parts of hisH substrate (thioester) and PRFAR • Today’s lab exercise will walk you through the parameterization of the hisH substrate

  30. HisH Mechanism • HisH glutamine amidotransferase • Conserved catalytic triad: C84, H178, E180

  31. HisH Mechanism • HisH glutamine amidotransferase • Conserved catalytic triad: C84, H178, E180

  32. Today’s Tutorial • VMD for introduction to the system • MOE to investigate glutamine approach to active site, make covalent bond • MOE will make initial approximation (guesses using pattern recognition) • Semi-empirical calculations with Spartan: • Geometry optimization, semi-empirical • Compare results of angles, bond lengths, etc. to what MOE guesses, experimental values • Torsional potential: dihedral drive • Minimization in NAMD2, look at results

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