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Designing a Syringe for Injectable Hydrogels

Designing a Syringe for Injectable Hydrogels. John Carradini Department of Biomedical Engineering Griffin Sonaty Department of Biomedical Engineering Faculty Mentor: David Zaharoff, Ph.D. Department of Biomedical Engineering 4 th Annual FEP Honors Research Symposium 8 November 2011.

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Designing a Syringe for Injectable Hydrogels

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  1. Designing a Syringe for Injectable Hydrogels John Carradini Department of Biomedical Engineering Griffin Sonaty Department of Biomedical Engineering Faculty Mentor: David Zaharoff, Ph.D. Department of Biomedical Engineering 4th Annual FEP Honors Research Symposium 8 November 2011

  2. Carradini, JP and GT Sonaty 4th Annual FEP Honors Research Symposium Cancer • According to the USDHHS, cancer will attack 1 out of every 2 men, and 1 out of every 3 women in America[1] • One of the leading causes of cancerous death is metastasis[2] • We need to find new treatments that eliminate the possibility of metastasis

  3. Carradini, JP and GT Sonaty 4th Annual FEP Honors Research Symposium Interleukin-12 However, there is one problem with IL-12:

  4. Carradini, JP and GT Sonaty 4th Annual FEP Honors Research Symposium IL-12 and Chitosan • IL-12 is toxic to the human body[3] • Chitosan is viscous, and can form a hydrogel at a certain pH • We can mix these to localize the distribution of IL-12 within the confines of the cancerous tumor • Due to viscosity, we must form the hydrogel within the body

  5. Carradini, JP and GT Sonaty 4th Annual FEP Honors Research Symposium Precipitating Chitosan • Chitosan will precipitate at a certain pH, forming a hydrogel • Start with Chitosan dissolved in HCl (acidic) • Add NaOH (basic) to raise the pH • As pH becomes less acidic, Chitosan becomes more likely to precipitate

  6. Carradini, JP and GT Sonaty 4th Annual FEP Honors Research Symposium Delivery Device • Syringe • Must be minimally-invasive • Must be non-reusable • Must keep Chitosan/IL-12 solution in HCl as a liquid while in the device • Must inject Chitosan/IL-12 solution in HCl and NaOH simultaneously at a certain ratio to reach specified pH within the tumor

  7. Carradini, JP and GT Sonaty 4th Annual FEP Honors Research Symposium Research Objectives • Find the pH and concentration levels at which Chitosan precipitates (Sigma, then Low-tox) • Find the ratio to mix NaOH and Chitosan/IL-12 solution in HCl in order to reach proper pH • Design and create a minimally invasive device to directly inject NaOH and Chitosan/IL-12 solution in HCl into a cancerous tumor • Test device and solution on mice (if able)

  8. Carradini, JP and GT Sonaty 4th Annual FEP Honors Research Symposium Schedule • We plan to meet in the Engineering Research Center at least on a bi-weekly basis • We will work collaboratively on the project, providing two perspectives on each aspect of the project • We will work together on the deliverables also, well in advance of deadlines

  9. Carradini, JP and GT Sonaty 4th Annual FEP Honors Research Symposium References • [1] United States Department of Health and Human Services (2011). 12th Report on Carcinogens. Introduction; p. 3. [Cited 3 November 2011] Available from: http://www.knovel.com/web/portal/browse/display?_EXT_KNOVEL_DISPLAY_bookid=4150&VerticalID=0 • [2] Personal Interview with David Zaharoff, Ph.D. on 30 September, 2011 • [3] Cohen, J. (1995). “Clinical Trials: IL-12 Deaths: Explanation and a Puzzle”, Science, Vol. 270, No. 5238, pp. 908. [Cited 3 November 2011] Available from: http://www.sciencemag.org/content/270/5238/908.1.short

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