1 / 37

Corrosion Cast (old imaging technique)

Corrosion Cast (old imaging technique). Systematic SEM imaging of Corrosion Casts What are Corrosion Casts? Negative “models” of the vascular system made by perfusion with a resin and following removal of tissue. Systematic SEM imaging of Corrosion Casts What are Corrosion Casts?

stan
Download Presentation

Corrosion Cast (old imaging technique)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Corrosion Cast(old imaging technique)

  2. Systematic SEM imaging of Corrosion Casts • What are Corrosion Casts? • Negative “models” of the vascular system made by perfusion with a resin and following removal of tissue

  3. Systematic SEM imaging of Corrosion Casts • What are Corrosion Casts? • Negative “models” of the vascular system made by perfusion with a resin and following removal of tissue • Assignment of areas by matching the sample to the monkey atlas figures

  4. Systematic SEM imaging of Corrosion Casts • What are Corrosion Casts? • Negative “models” of the vascular system made by perfusion with a resin and following removal of tissue • Assignment of areas by matching the sample to the monkey atlas figures • Intention for new cast preparations: • Perfusion with cast medium was optimized • Large samples from all brain regions can now be obtained • Descriptive part for planned Atlas of Angioarchitecture

  5. Corrosion Cast Gallery 1

  6. V1

  7. V1

  8. V1, cuffs

  9. V1, pial surface

  10. V2

  11. V3

  12. TEO, V4 ios

  13. sts, large collecting vein

  14. Artery, Vein

  15. Ventricle

  16. Corrosion Cast(new imaging technique)

  17. Corrosion Cast • Serial slabs from a whole quadrant • Maceration of the complete slab • Surface-matching to the anatomical atlas • Reliable determination of certain brain areas • High resolution imaging of entire samples • Zeiss Supra EM + multiscan software  Novel visualization quality of the cortical angioarchitecture

  18. 1 cm

  19. Offline determination of arteries and veins • Estimation of a/v-ratio • ~1:1.6 with more arteries • No regional differences of this ratio observed so far…

  20. Striate cortex Extra-striate cortex .5 mm .5 mm V1 V2 • …whereas regional differences in density as already quantified by immunohistochemistry are present and also visible in the cast preparation

  21. 1 cm

  22. 7a

  23. MT

  24. MST

  25. V3a

  26. Outlook • Preparation of horizontal and coronal slabs of the remaining three hemispheres • Systematic investigation of certain cortical and subcortical areas throughout the brain • Descriptive part of a long-term planned „Atlas of the Macaque‘s Angioarchitecture“

More Related