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Genetic toggle switch: a biochemical RS latch

RS latch (bistable). Genetic toggle switch (bistable). Genetic toggle switch: a biochemical RS latch. Electronic memory. Biochemical memory. Repressible promoter. Transistor. Contruction of the genetic toggle switch in E. coli plasmid.

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Genetic toggle switch: a biochemical RS latch

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  1. RS latch (bistable) Genetic toggle switch (bistable) Genetic toggle switch: a biochemical RS latch Electronic memory Biochemical memory Repressible promoter Transistor

  2. Contruction of the genetic toggle switch in E. coli plasmid Collaboration with James Collins & Charles Cantor, Boston University

  3. The toggle exhibits bistable (“sticky”) switching Grey Shading: Application of IPTG Split culture into two cultures

  4. 0 0 Toggle model identifies the minimal conditions for bistability Nonlinear ODE model: reduced rate equations for transcription and translation CI lacR

  5. Weak cI or tetR repressor Imbalanced promoters repressors lead to monostability (GFP) Single stable state

  6. Toggles are bistable for variety of promoter strengths and types LacI/TetR Toggle Imbalanced promoters destroy bistability

  7. Results: Switching Time Switching ON Switching OFF

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