Environmental Professional Contracting Services

1. EPCS Environmental LLC Environmental Professional Contracting Services Dale Anderson EPCS Environmental LLC July 1, 2008

2. EPCS Environmental LLC Biofilm New Health Risk

3. Kill Attacking Amoebae ScienceDaily (July 25, 2008) — Bacteria rarely come as loners; more often they grow in crowds and squat on surfaces where they form a community together. These so-called biofilms develop on any surface that bacteria can attach themselves to. The dilemma we face is that neither disinfectants and antibiotics, nor phagocytes and our immune system can destroy these biofilms. • Amoebae normally attack and kill harmful pathogens • Pathogens have become Resistant (biofilm bacteria use chemical weapons to defend themselves.) • They clump together and build a slimy fortress of defense • Effective in the presence of all amoebae, killing them or causing them to become de-activated Common Problem Areas • Athletic Facilities • Hospitals • Implants • Catheters • Surfaces • Surgical equipment Environmental Professional Contracting Services

4. ÆGIS/mPale … Microbe Shield • Microbe Shield Program based on a unique antimicrobial technology which effectively controls a broad spectrum of bacteria, fungi, algae and yeasts on a wide variety of treated substrates • Used safely and effectively for 30 years • Tested and approved for use in EPCS electrostatics sprayers Environmental Professional Contracting Services

5. ÆGIS/mPale … Microbe ShieldThe Mark of Excellence Environmental Professional Contracting Services

6. ÆGIS/mPale Microbe Shield TreatmentStaphylococcus aureus ( MRSA ) Photographs taken by electronic microscope Not Treated Treated

7. ÆGIS/mPale … Microbe ShieldRepresentative Microorganisms Tested: A Partial Compendium Environmental Professional Contracting Services

8. ÆGIS/mPale … Microbe ShieldRepresentative Microorganisms Tested: A Partial Compendium (cont’d) Environmental Professional Contracting Services

9. The ÆGIS/mPale Microbe Shield • Highly Effective • Long-Lasting • Odorless/Colorless • Extremely Low Toxicity • Long History of Safe Use • Permanent Chemical Bonding • Won’t Wash Off or Affect Skin Microflora Environmental Professional Contracting Services

10. Cleaner, sanitizer and deodorizer Registered with the EPA as a germ killer and fungicide and is authorized for use in food preparation areas. It is specially formulated for compatibility with mPale Antimicrobial. Virucide Fungicide (against pathogenic fungi) Mildewstat (on hard, inanimate surfaces) Effective in the presence of 5% serum contamination For home, hospital, institutional and industrial use, school, restaurant, food handling and processing areas Kills germs mPerial™ Environmental Professional Contracting Services

11. ÆGIS/mPale § mPerial™ Treatment • All touch surfaces • Fountain area for drinks and ice • Carpet and floor mats • Doorknobs, handrails • Rest rooms • Play ground support products Environmental Professional Contracting Services

12. ÆGIS/mPale § mPerial™ Treatment • New construction • Ceilings • Floors • Walls • Wall cavities • Above ceiling space • Chases • Furnishings • A/C ducts

13. ÆGIS/mPale § mPerial™ Treatment • Transportation: Trucks and Trailers • Reduces bacteria load • Reduces odors • Makes treated surfaces easier to clean • Tremendous value to cost ratio • Tagging agent built-in to verify validity Environmental Professional Contracting Services

14. EPCS Environmental LLC Evaluation of a Silane Quarternary Ammonium Salt as Antimicrobial Fabric Treatment Arvye Davis, Randall Sumner, SLSTP-Flight Group2004

15. Overview Antimicrobial agents are used to kill or limit growth of microorganisms. ÆGIS Microbe Shield™, an antimicrobial agent composed of a silane quaternary ammonium salt, is featured for its durability and efficacy against both gram-negative and gram-positive bacteria. This research studied the effectiveness of the antimicrobial agent as a treatment for cotton fabric. Cotton fabric samples were coated with the antimicrobial agent, then tested against three species of bacteria. The samples were shaken in a bacterial solution to allow contact with the microbes, and the number of viable bacteria were counted. The data were compared to the numbers of bacteria with untreated fabric. This research reveals that the antimicrobial agent reduced the numbers of bacteria exposed to the treated cotton fabric. Environmental Professional Contracting Services

16. Research • The three types of bacteria were Escherichia coli, Staphylococcus epidermidis, and Bacillus subtilus. For each test, the bacteria were grown in Tryptic Soy Broth (TSB) for eighteen hours at room temperature. The bacteria were diluted in 0.003M KH2PO4 to 72 percent transmission using a Vitek Colorimeter. For testing, the bacteria were diluted 1:1000 to 1.5-3.0 x 105 cfu/mL. Then, 50mL aliquots of the diluted bacteria were distributed into sterile 250mL flasks. • The number of bacteria were determined by the pour plate method using Tryptic Soy Agar (TSA). The control was plated at “0” contact time. The nine flasks were agitated in a reciprocal shaker at 120 RPM. After one hour, all bacteria samples were plated and incubated for 24 hours at 35°C. Environmental Professional Contracting Services

17. Results It was discovered that the antimicrobial agent did exhibit the ability to kill the three species of bacteria. Graph 1: Comparison of all pure cultures used with and without the antimicrobial agent. Acknowledgements This research was conducted as part of the 2004 Spaceflight and Life Sciences Training Program funded by the National Aeronautics and Space Administration. The authors recognize the support of the Dynamac Corporation, the NASA Spaceflight and Life Sciences Training Program Academic Partner Alliance and the United States Department of Agriculture. I would like to thank my Principal Investigator, Randall Sumner, for his hard work in preparation of this research, and I would also like to thank Jason Johns and Adam Santone for their help and guidance in this research. Environmental Professional Contracting Services

18. EPCS Environmental LLC Electrostatic Spraying Technology

19. EPCS Environmental LLC Inefficiencies of Conventional Sprayers

20. Inefficiencies of conventional sprayers • Of the total amount of product applied with conventional sprayers • 60% to 70% goes on the floor/ground • 15% to 20% drifts from immediate area • only 15 to 20% is deposited on the target • less than 3% contacts near the intended area under target on target drifts floor/ground Environmental Professional Contracting Services

21. EPC EPCS Environmental LLC Advantages Electrostatic Technology

22. Air-Assisted Electrostatic Sprayers • The charged droplets seek out surfaces by electrostatic forces. The result is a uniform spray coverage in hiddenareas: underside, crevices, holes, and empty spaces you can’t see with a naked eye. • RESULTS: is over 30 to 45-fold increase to these hidden areas. Environmental Professional Contracting Services

23. Using electrical charges to enhance the behavior of spray OTHER BENEFITS: Over 4 to 7-fold deposit efficiency improvement Provides uniform dispersion and coating Leaves no visible residue Reduces “hang-time” Coats air-borne and non-conductive targets Environmental Professional Contracting Services

24. BENEFIT: Reduces “Hang-Time” 1/30th of a second Uncharged Spray Charged Spray Charged droplets move much faster to the target Environmental Professional Contracting Services

25. Demonstration of Electrostatic Coating on Non-conductive Target BENEFIT: Reduces “hang-time”;Coats air-borne and non-conductive targets Uncharged spray Charged spray Environmental Professional Contracting Services

26. EPCS Environmental LLC Electrostatics Demo “Black Ball”

27. Black Ball Demo First, we spray the sphere with electrostatics turned OFF: 1 – 2 – 3 – 4 seconds. (The target object was sprayed from a distance of 3 meters; the black background was added to make the spray more visible.) Environmental Professional Contracting Services

28. Black Ball Demo Notice the coverage on the target. Environmental Professional Contracting Services

29. Black Ball Demo Finally, we turn electrostatics ON, and spray a new sphere for half the time: 1 – 2 seconds. (All other settings are kept constant, so half the amount of material is sprayed.) Environmental Professional Contracting Services

30. Black Ball Demo Notice the coverage on the target. The charged droplets wrapped around, reaching the back of the sphere. Environmental Professional Contracting Services

31. Black Ball Demo Results Original Sphere Electrostatics OFF Electrostatics ON Environmental Professional Contracting Services

32. EPCS Environmental LLC Successful Applications Healthcare, Athletics/Sports Facilities, Food Industry, Correctional Facilities, Airline Industry, Schools/Colleges, Residential/Businesses

33. Control of Microorganisms in Indoor Environments • Medical Centers / Hospitals / Hospices • DEMO • Athletics / Sports Facilities • Virginia Tech University • Correctional Facilities • Metro Detention Center • Airline Industry • Schools / Colleges • Over 75 school coated in 2006 & 2007 • Residential / Businesses • Mohawk - World’s largest floor manufacturer EXAMPLES Environmental Professional Contracting Services

34. Case StudyArthur G. James Cancer Hospital & Research Institute, Columbus, OHLee Memorial Hospital, Lee County, FL Improved Control of Microbial Exposure in Hospitals: A 30 Month Field Study

35. Case StudyWashington Redskins, Washington, D.C. Over 26 sports facilities coating in the last 22 months. Environmental Professional Contracting Services

36. Case StudyMetro Detention CenterAtlanta, GA Environmental Professional Contracting Services

37. Case StudyAirlines Industry Test coating cabins of two 757’s Environmental Professional Contracting Services

38. Various Institutions: • Virginia Tech • Ohio State University • University of South Carolina • University of Tulsa • Southern Illinois University • University of Illinois Southern • Missouri State • SwathmoreCollage • Atlanta Thrashers • Cal State Bakersfield • Stevens Institute of Technology • Columbia University • Grundy Wrestling • John Hopkins University Environmental Professional Contracting Services

39. Various Sports Facilities: • Miami Heat • Cleveland Browns • Washington Wizards • Atlanta Hawks • New Jersey Nets • Washington Redskins • Minnesota Vikings • Detroit Lions Environmental Professional Contracting Services

40. The WOW F actors • Electrostatic technology = The 1st Wow! • mPale § mPerial products = The 2nd Wow! • Ability to measure the amount of mPale on the surface/target = The 3rd Wow! $15M research was invested to discover these Wows! Environmental Professional Contracting Services

41. Three Step Game Plan • You need a plan designed to protect your sports facilities, equipment and synthetic turf fields. • The big advantages to the Three Step Game Plan • Less Invasive • More Effective • Faster • Lower Cost • Longer Lasting • More Versatile • More Efficient • Moisture Resistant Environmental Professional Contracting Services

42. Three Step Game Plan • Sophisticated Technologies • Advanced Application Protocols • More Affordable Than Ever • What's your game plan? Environmental Professional Contracting Services

43. EPCS