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Microtubules and Cilia: Structure and Function in Living Cells

Explore the dynamic instability of microtubules, the structure and beating movement of cilia and flagella, and the role of cilia in development and disease. Understand how proteins regulate microtubule growth and sliding movements, and the importance of intraflagellar transport in assembling and maintaining flagella. Discover the mechanism behind ciliary and flagellar locomotion, as well as the impact of mutations on ciliary proteins in human health. Dive into the assembly, disassembly, types, and functions of intermediate filaments and microfilaments in cell motility and structural support.

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Microtubules and Cilia: Structure and Function in Living Cells

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  1. Microtubules (17) • Dynamic instability • Growing and shrinking microtubules can coexist in the same region of a cell. • A given microtubule can switch back and forth between growing and shortening phases. • It is an inherent property of the plus end of the microtubule. • Proteins called +TIPS regulate the rate of growth and shrinkage.

  2. Microtubule dynamics in living cells

  3. Dynamic instability

  4. Microtubules (18) • Cilia and Flagella: Structure and Function • Cilia and flagella are hairlike motile organelles. • They have similar structures but different motility. • Cilia tend to occur in large numbers on a cell’s surface.

  5. Beating movement of cilia

  6. Beating movement of cilia

  7. Microtubules (19) • Cilia and flagella (continued) • Flagella exhibit different beating patterns. • The structure of cilia and flagella contains a central core (axoneme) consisting of microtubules in a 9 + 2 arrangement.

  8. Eukaryotic flagella

  9. Microtubules (20) • Cilia and flagella (continued) • The basic structure of the axoneme includes a central sheath, connected to the A tubules of peripheral doublets by radial spokes. • The doublets are interconnected to one another by an interdoublet bridge. • A longitudinal view of the axoneme shows the continuous nature of the microtubules

  10. The structure of the axoneme

  11. Longitudinal view of an axoneme

  12. Microtubules (21) • Cilia and flagella (continued) • Cilia and flagella emerge from basal bodies. • The growth of an axoneme occurs at the plus ends of microtubules. • Intraflagellar transport (IFT) is the process responsible for assembling and maintaining flagella. • IFT depends on the activity of both plus end- and minus end-directed microtubules.

  13. Basal bodies and axonemes

  14. Intraflagellar transport

  15. Microtubules (22) • The Dynein Arms • The machinery for ciliary and flagellar motion resides in the axoneme. • Ciliary (axonemal) dynein is required for ATP hydrolysis, which supplies energy for locomotion.

  16. Chemical dissection of protozoan cilia

  17. A model of structure and functionof ciliary dynein

  18. A model of structure and functionof ciliary dynein

  19. Microtubules (23) • The Mechanism of Ciliary and Flagella Locomotion • Swinging cross-bridges generate forces for ciliary or flagellar movement. • Dynein arm of an A tubule binds to a B tubule and undergoes a conformational change that slides tubules past each other. • Sliding alternates from one side of axoneme to another leading to bending.

  20. Forces that drive ciliary or flagella motility

  21. The Human Perspective: The Role of Cilia in Development and Disease (1) • Situs inversus is a syndrome in which the left-right body symmetry is reversed. • One cause of situs inversus is mutations in the gene encoding ciliary proteins. • Patients with situs inversus suffer from respiratory infections and male infertility.

  22. The Human Perspective: The Role of Cilia in Development and Disease (1) • Many cells have nonmotile primary cilia that sense chemical and mechanical properties of surrounding fluids. • Mutations in primary cilia may lead to polycystic kidney disease. • Cilia are important in developmental processes, and mutations lead to a range of abnormalities.

  23. Primary cilia

  24. 9.4 Intermediate Filaments (1) • Intermediate filaments (IFs)– heterogeneous group of proteins, divided into five major classes. • IFs classes I–IV are used in the construction of filaments; type V (lamins) are present in the inner lining of the nucleus.

  25. Distribution of major mammalian IF proteins

  26. Intermediate Filaments (2) • IF Assembly and Disassembly • Assembly: • Basic building block is a rod-like tetramer formed by tow antiparallel dimers. • Both the tetramer and the IF lack polarity. • IFs are less sensitive to chemical agents than other types of cytoskeletal elements.

  27. A model of IF assembly and architecture

  28. Intermediate Filaments (3) • Assembly and disassembly of IFs are controlled by phosphorylation and dephosphorylation

  29. Intermediate Filaments (4) • Types and Functions of IFs • IFs containing keratin form the protective barrier of the skin, and epithelial cells of liver and pancreas. • IFs include neurofilaments, which are the major component of the network supporitng neurons.

  30. Organization of IFs within an epithelial cell

  31. 9.5 Microfilaments (1) • Microfilaments are composed of actin and are involved in cell motility. • Using ATP, actin polymerizes to form actin filaments (“F-actin”). • The two ends of an actin filament have different structural characteristics and dynamic properties.

  32. Actin filament structure

  33. Microfilaments (2) • One of the micro-filaments appears pointed, and the other appears barbed. • Orientation of the arrowheads formed by actin provides information about direction of the microfilament movement.

  34. Microfilaments (3) • Microfilament Assembly and Disassembly • Actin assembly/disassembly in vitro depends upon concentration of actin monomers. • Filament assembly leads to drop in ATP-actin. • Actin subunits are added to plus end and removed from the minus end (steady state). • Microfilament cytoskeleton is organized by controlling equilibrium between assembly and disassembly of microfilaments.

  35. Actin assembly in vitro

  36. Microfilaments (4) • Actin polymerization can act as a force-generating mechanism in some cells.

  37. Microfilaments (5) • Myosin: The Molecular Motor of Actin Filaments • All myosins share a characteristic motor head for binding actin and hydrolyzing ATP. • The myosin tail is divergent. • Myosins can be divided into two groups: • Conventional (type II) myosins • Unconventional myosins

  38. Microfilaments (6) • Conventional (Type II) Myosins • They generate force in muscles and some nonmuscle cells. • Each myosin II is composed of two heavy chains, two light chains, and two globular heads (catalytic sites).

  39. Structure of myosin II

  40. Microfilaments (7) • Myosin II (continued) • All of the machinery required for motor activity is contained in a single head. • The tail portion plays a structural role allowing the protein to form filaments.

  41. Myosin II

  42. Myosin II

  43. Microfilaments (8) • Unconventional Myosins • They have only a single head and are unable to assembly into filaments in vitro. • Myosin I’s precise role in cellular activities is unclear. • Myosin V is involved in organelle transport. • Several of them are associated with cytoplasmic vesicles and organelles.

  44. Myosin V and organelle trasnport

  45. Unconventional myosins inintracellular transport

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