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Collection of Blood from Cardiac puncture in Rat

General anesthesia needed Large amount: up to 3% of body weight. Collection of Blood from Cardiac puncture in Rat. 75% alcohol cotton ball for surface disinfection 24G needle with 10cc syringe for blood withdraw 15 cc centrifuge tube for blood collection.

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Collection of Blood from Cardiac puncture in Rat

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  1. General anesthesia needed Large amount: up to 3% of body weight Collection of Blood from Cardiac puncture in Rat

  2. 75% alcohol cotton ball for surface disinfection 24G needle with 10cc syringe for blood withdraw 15 cc centrifuge tube for blood collection Tools for collection of blood from cardiac puncture

  3. Disinfect the left thorax with 75% alcoholic cotton ball

  4. Search for the point of maximum heart beat

  5. Insert the needle straight on the selected point and withdraw blood by your left hand

  6. http://www.jove.com/index/Details.stp?ID=266

  7. Feeding amount limited to 1% of body weight Oral Feeding in Rat

  8. 16 G ball-tipped feeding needle with syring Leather glove Tools for oral feeding in rat

  9. Restrain the shoulders of the rat by your thumb and index finger, then support the lower limbs with your right hand

  10. Restrain the tail of the rat in between your ring finger and little finger

  11. Let the rat lying on your left palm and introduce the ball-tipped feeding needle from the pharynx in to the esophagus when the rat is in the act of swallowing

  12. SEDATION AND TRANQUILIZATION CHEMICAL RESTRAINT

  13. Mild tranquilizer Dose: 0.5 – 2.5 mg/kg IM or SQ Lower dose for IM Higher dose for SQ ACEPROMAZINE

  14. Mild sedation Dose: 3-5 mg/kg IM IM injection may be irritating to muscle DIAZEPAM

  15. Excellent sedative Can see narcotic induced respiratory depression with higher doses Primarily used in rats and mice Doses Rats 0.13 – 0.33 mg/kg IM Provides sedation and anesthesia for minor procedures FENTANYL/DROPERIDOL

  16. Used as a premed Decrease salivation (Ketamine, inhalants) Maintain heartrate Atropine 0.05 mg/kg SQ Glycopyrrolate 0.01 – 0.02 mg/kg SQ ANTICHOLINERGICS

  17. INJECTABLE ANESTHESIA Clinical Laboratory Animal Medicine book Page-91 CHEMICAL RESTRAINT

  18. Short duration of action (30 minutes) Rats Xylazine: 5-10 mg/kg + Ketamine: 40-80 mg/kg IM or IP Mice Xylazine: 5-10 mg/kg + Ketamine: 50-200 mg/kg IM or IP XYLAZINE/KETAMINE

  19. Adequate restraint for minor surgical procedures General Rule: the smaller the species, the greater the dose needed for adequate effect Rats and Mice Acepromazine: 2.5-5 mg/kg + Ketamine 75-80 mg/kg IM or IP KETAMINE/ACEPROMAZINE

  20. For minor surgical procedures Dose 20-40 mg/kg IM or IP Can have prolonged recovery Used in combination with xylazine Decrease dose to 5-10 mg/kg Better effect and faster recovery TILETAMINE-ZOLAZEPAM

  21. Use for any major surgical procedure Induce with gas alone or with injectable anesthetics Isoflurane is the agent of choice INHALANT ANESTHESIA

  22. Birchard, S.J., & Sherding, R.G. (2000). Saunders Manual of Small Animal Practice, Second Edition. Phildephia, PA. WB Saunders Company. Fowler, M.E. (1995). Restraint and Handling of Wild and Domestic Animals, Second Edition. Ames, IA. Iowa State University Press. Frye, F.L. (1994) Reptile Clinician's Handbook: a compact clinical and surgical reference. Krieger Pub Co. Beynon, P.H. (1992) Manual of reptiles. Cheltenham, Gloucestershire. British Small Animal Veterinary Association. Hillyer, E.V., & Quesenberry, K.E. (1997). Ferrets, Rabbits and Rodents: Clinical Medicine and Surgery. Philedephia, PA. WB Saunders Company REFERENCES

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