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Daria Bedulina Laboratory of biosystem adaptation Irkutsk State University

Modification of MXR-activity in freshwater amphipods Eulimnogammarus cyaneus and E. verrucosus by organic and inorganic contaminants. Daria Bedulina Laboratory of biosystem adaptation Irkutsk State University Adviser: Dr M. Timofeev. environment. environment. STRESS. ADAPTATION.

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Daria Bedulina Laboratory of biosystem adaptation Irkutsk State University

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  1. Modification of MXR-activity in freshwater amphipods Eulimnogammarus cyaneus and E. verrucosus by organic and inorganic contaminants. Daria Bedulina Laboratory of biosystem adaptation Irkutsk State University Adviser: Dr M. Timofeev

  2. environment environment STRESS ADAPTATION Organism

  3. The organisms stress adaptation levels: • Behavioral • Physiological • Biochemical and molecular • Multixenobiotic resistance system (MXR)

  4. Multixenobiotic resistance system (MXR) P-glycoprotein (P-gp)

  5. Method of MXR-activity definition: Rhodamine B efflux method

  6. LAKE BAIKAL

  7. Lake Baikal Fauna >> 2500 species (60-80% endemics) Amphipods (Crustacea, Amphipoda) More than 250 speciesandsubspecies

  8. Contaminants, used in research Humic substances • Organic contaminant Natural organic matter (NOM) • Inorganic contaminant CdCl2

  9. The objects of research: Eulimnogammarus verrucosus(Gerstf.) E.cyaneus(Dyb.) 2

  10. Rhodamine B-efflux method P-gp Accumulation

  11. Rhodamine B-efflux method P-gp Efflux

  12. Results MXR-activity in amphipods without contaminants (control) (Rhodamine B efflux) Eulimnogammarus cyaneus Eulimnogammarus verrucosus

  13. Results MXR-activity in amphipods, NOM solutions (60 mg/ml) (Rhodamine B efflux) Eulimnogammarus cyaneus Eulimnogammarus verrucosus control NOM

  14. Results MXR-activity in amphipods CdCl2 solution (2.2 µmol/ml.) (Rhodamine B efflux) Eulimnogammarus cyaneus Eulimnogammarus verrucosus control CdCl2

  15. Discussion • Baikalian amphipods use a MXR system for xenobiotics efflux. • Activity of this system is inhibited by some organic and inorganic contaminants.

  16. Conclusion The multixenobiotic resistance mechanism in baikalian organisms is sensitive to organic and inorganic contaminants, such as heavy metal and NOM.

  17. This research was partially supported by grants of Russian Foundation for Basic Research No. 05-04-97239-Baikal, 05-04-97263-Baikal and 06-04-48099 The presented results was published Timofeyev M.,  Shatilina Z.M., Bedulina D.S., Menzel, R., Steinberg C.E.W. Natural organic matter (NOM) has the potential to modify the multixenobiotic resistance (MXR) activity in freshwater amphipods Eulimnogammarus cyaneus (Dyb) and Eulimnogammarus verrucosus (Gerst.)// Comparative biochemistry and physiology, Part B 146 (2007) 496–503 

  18. Thank you!

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