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Oregon State University Seed laboratory

Oregon State University Seed laboratory Feasibility of Discontinuing the Factoring Procedure in Fine Fescues. AOSA Requires to Factor the Following Fine Fescues. Hard fescue. Sheep fescue. Chewing fescue. Creeping red fescue. Factoring applies these percentages.

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Oregon State University Seed laboratory

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  1. Oregon State University Seed laboratory Feasibility of Discontinuing the Factoring Procedure in Fine Fescues

  2. AOSA Requires to Factor the Following Fine Fescues • Hard fescue. • Sheep fescue. • Chewing fescue. • Creeping red fescue.

  3. Factoring applies these percentages

  4. Problems Associated with Factoring Fine Fescues • It is subjective: can change from analyst to analyst. • It is time consuming: MSU’s must be: - Separated, - Weighed, -Run calculations. Then the MSU’s have to be mixed back with single florets for germination test. • Harmonization issue: with ISTA. • It puts USA seed producers at a disadvantage in the international markets.

  5. Factoring requires these Separations

  6. Better alternative!Consider multiple floret as a pure seed unit, if it has a fertile floret (it has a planting value!).There are many benefits for this approach supported by research findings

  7. Three main research studies 1. Preliminary research at OSU. 2. Research with data from 3 Northwest Labs. 3. National referee with 9 laboratories.

  8. 1. Preliminary Study at OSU4 fine fescue species - 2 samples each.Objectives- Compare the % pure seed with & without factoring procedure. - Measure the % germination of single and multiple seed units. - Measure time saving.

  9. Materials & Methods 2 samples of Hard, sheep, chewing, and creeping red fescues from different varieties and production years containing 50-300 MSU’s. Procedure:% Purity was calculated first by factoring according to the AOSA rules and then without factoring (consider MSU’s as pure seeds). Germination tests: of MSU vs. SSU: seeds were chilled at 10°C for 7d. Germination % was reported after 14 days.

  10. Pure seed % of 8 F.F. samples tested with and without applying Factoring Procedure Avg. diff 0.49%)

  11. Germination of single and multiple seed units of 8 F. F. samples Avg. diff 1%

  12. Germination of Single and Multiple Seed Units in Fine Fescue Multiple Seed Units Single Seed Units

  13. Conclusions of the Preliminary Study • The average difference in purity results between factoring and no-factoring is 0.49 • Multiples that have fertile florets have similar germination capacity as single seed units. • MSU have planting value and should be considered pure seed units without factoring.

  14. Study 2. Comparison of factoring and no-factoring using data from 3 NW Labs Materials and methods • 3 labs participated in the referee, WA, AgriSeed, and OR. • A total of 659 samples representing 4 FF species were used by all labs from different production years: • 159 chewing, 145 hard, 88 sheep, and 267 creeping red samples. • All samples tested with and without applying the factoring procedure across the 3 labs. • Average pure seed % were recorded and compared between the two methods.

  15. I. AgriSeed Testing

  16. I. AgriSeed Testing

  17. I. AgriSeed Testing Sheep fescue, 2011

  18. I. AgriSeed Testing

  19. I. AgriSeed Testing

  20. I. AgriSeed Testing

  21. Creeping red fescue 2010

  22. II. Oregon state University

  23. II. Oregon state University

  24. II. Oregon state University

  25. II. Oregon state University

  26. II. Oregon state University

  27. II. Oregon state University

  28. II. Oregon state University

  29. III. WA State Seed Lab

  30. III. WA State Seed Lab

  31. III. WA State Seed Lab

  32. III. WA State Seed Lab

  33. III. WA State Seed Lab

  34. III. WA State Seed Lab Avg. diff 0.91%

  35. Conclusions from Northwest Labs Discontinuing factoring would increase purity by an average of 0.53% (average of 659 samples). The average increase in each species is: • 0.38% for hard fescue ( 145 samples ). • 0.45% for sheep fescue ( 88 samples ). • 0.63% for chewing fescue ( 159 samples ). • 0.67% for creeping red fescue ( 267 samples ).

  36. 3. National Referee Objectives • Compare the purity results of factoring vs. no-factoring across laboratories. • Measure consistency of both methods across labs. • Measure the time saving with the no-factoring method.

  37. Materials & Methods • 11 different analysts representing 9 seed labs participated in the study from AR, USDA-FSL, BioVision, KY, NST, Turf Tech, WA, and OR. • Labs were asked to compare the purity results of 8 FF samples representing creeping, chewing, hard and sheep fescue, with different varieties, years of production, and MSU contents, using factoring and no-factoring procedures. • Labs were also asked to measure the time used to complete each test using factoring and non-factoring methods. • Data were collected to measure the consistency of both methods across laboratories.

  38. Results

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