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HSPA4L

MCA clone 273bp. HSPA4L. HSPA4L. CPG sites. TSS. CpG. -21. Pyrosequencing. Raji (99%). C=100% T=0%. C=100% T=0%. C=98% T=2%. Raji M%=99. Control (0%). C=2% T=98%. C=1% T=99%. C=1% T=99%. Control M%=1. ALL (89%). ALL M%=96. C=90% T=10%. C=100%

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HSPA4L

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  1. MCA clone 273bp HSPA4L HSPA4L CPG sites TSS CpG -21 Pyrosequencing Raji (99%) C=100% T=0% C=100% T=0% C=98% T=2% Raji M%=99 Control (0%) C=2% T=98% C=1% T=99% C=1% T=99% Control M%=1 ALL (89%) ALL M%=96 C=90% T=10% C=100% T=0% C=96% T=4% Supplemental Figure 1 a c b Figure 1: Validation of HSP4AL methylation in Raji, normal control and an ALL samples a. Representation of HSPA4L: CpG sites are indicated by short vertical bars. Exon by black rectangle on top, and the position of the MCA/RDA clone by gray rectangles. The size of the clone is also indicated. Arrow points to transcription start site. Pyrosequencing primer position is shown grey box below. b. Representative pyrograms of HSPA4L: 3 CpG sites near the transcription start site were bisulfite pyrosequenced, and a pattern of high levels of methylation (M%) was observed for all of them in Raji, and ALL sample but no in the control c. Methylation analysis of HSPA4L by bisulfite sequencing. The entire CPG islands was densely methylated in Raji and ALL sample but not in control sample. Each row of circles represents the sequence of an individual clone. Open circles, unmethylated CpG sites; filled circles, methylated CpG sites.TSS, transcription start site. Methylation density was calculated as the average value of the 10 clones’ methylation density. Bisulfite sequencing results were consistent with bisulfite pyrosequencing.

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