Meiosis and Sexual Reproduction Chapter 10

1. Meiosis and Sexual ReproductionChapter 10

2. Outline • Reduction in Chromosome Number • Meiosis Overview • Homologous Pairs • Phases of Meiosis • Meiosis I • Meiosis II • Meiosis Compared to Mitosis • Genetic Variation • Crossing-Over • Independent Assortment • Fertilization • Human Life Cycle

3. Discovery of Meiosis • Meiosis was first observed by the Belgian cytologist Pierre-Joseph van Beneden in 1887 • Gametes (eggs and sperm) contain half the number of chromosomes found in other cells (haploid) • The fusion of gametes is called fertilization • It creates the zygote, which contains two copies of each chromosome (diploid)

4. Contain one set of chromosomes • Sexual reproduction • Involves the alternation of meiosis and fertilization • Asexual reproduction • Does not involve fertilization Contains two sets of chromosomes

5. The Sexual Life Cycle in Animals

6. Meiosis:Halves the Chromosome Number • Special type of nuclear division • Used only for sexual reproduction • Halves the chromosome number prior to fertilization • Parents diploid • Meiosis produces haploid gametes • Gametes fuse in fertilization to form diploid zygote • Becomes the next diploid generation

7. Homologous Pairs of Chromosomes • In diploid body cells chromosomes occur in pairs • Humans have 23 different types of chromosomes • Diploid cells have two of each type • Chromosomes of the same type are said to be homologous • They have the same length • Their centromeres are positioned in the same place • One came from the father (the paternal homolog) the other from the mother (the maternal homolog) • When stained, they show similar banding patterns • Because they have genes controlling the same traits at the same positions

8. Homologous Chromosomes

9. The Stages of Meiosis • Meiosis consists of two successive divisions, but only one DNA replication • Meiosis I • Separates the two versions of each chromosome (homologous chromosomes) • Meiosis II • Separates the two sister chromatids of each chromosome • Meiosis halves the number of chromosomes

10. Overview of Meiosis

11. Meiosis I (Reductional Division) • Prophase I • Nuclear membrane breaks down • Homologous chromosomes pair up and exchange segments (crossing over) • Metaphase I • Homologous chromosome pairs align at random in the equatorial plane such that maternal or paternal member may be oriented toward either pole (independent assortment) • Anaphase I • Homologous chromosomes (each still consisting of 2 chromatids) separate and move to opposite poles • Telophase I • Individual chromosomes gather together at each of the two poles • Cytokinesis produces 2 daughter cells which are haploid

12. Interkinesis Meiosis I

13. Crossing Over Meiosis I Prophase I • The longest and most complex stage of meiosis • Homologous chromosomes undergo synapsis • Pair up along their lengths • Crossing overoccurs

14. Independent Assortment • Meiosis I • Metaphase I

15. Independent Assortment • In humans, a gamete receives one homologue of each of the 23 chromosomes • Humans have 23 pairs of chromosomes • 223 combinations in an egg or sperm • 8,388,608 possible kinds of gametes Three chromosome pairs 23 combinations

16. Meiosis II • After meiosis I there is a brief interphase (interkinesis) • No DNA synthesis occurs • Meiosis II is similar to mitosis, but with two main differences • 1. Haploid set of chromosomes • 2. Sister chromatids are not identical

17. Prophase II • Brief and simple, unlike prophase I • Cells have 1 member of each homologous pair • Metaphase II • Chromosomes line up at the equator • Anaphase II • Spindle fibers contract, splitting the centromeres • Sister chromatids move to opposite poles • Telophase II • Nuclear envelope reforms around four sets of daughter chromosomes • Cytokinesis occurs • Meiosis II

18. Interkinesis Meiosis I

19. No two cells are alike Meiosis II

20. Overview of meiosis • 2 divisions, 4 daughter cells (not identical) • Cells are diploid at beginning of meiosis • Pairs of chromosomes are called homologues (homologous chromosomes) • Meiosis I • Homologues line up side by side at equator-synapsis • When pairs separate, each daughter cell receives one member of the pair • Cells are now haploid • Meiosis II • No replication of DNA occurs in this division • Centromeres divide and sister chromatids migrate to opposite poles to become individual chromosomes • Each of the four daughter cells produced has the haploid chromosome number and each chromosome is composed of one chromatid

21. Genetic Variation: Crossing Over and Independent Assortment • Meiosis I brings about genetic variation in two key ways: • Crossing over-exchange of segments of DNA between homologues (Prophase I) • Independent assortment of chromosome pairs (Metaphase I) • When homologues align at the metaphase plate • They separate in a random manner • The maternal or paternal homologue may be oriented toward either pole of mother cell • Promotes genetic variability • Both assure that gametes will contain different combinations of chromosomes • When fertilization occurs, the resulting offspring will genetically unique

22. In comparison of meiosis to mitosis note that: • DNA replication occurs only once prior to both • Meiosis requires 2 divisions, mitosis only 1 • Meiosis produces 4 daughter cells, mitosis produces 2 • Daughter cells from meiosis are haploid, those from mitosis are diploid • Daughter cells from meiosis are genetically unique, while those from mitosis are genetically identical

23. Comparing Meiosis and Mitosis • Meiosis and mitosis have much in common • However, meiosis has two unique features • 1. Crossing over • Homologous chromosomes pair all along their lengths in meiosis I and exchange pieces of DNA • 2. Reduction division • There is no chromosome duplication between the two meiotic divisions • This produces haploid gametes

24. Meiosis Compared to Mitosis

25. Meiosis Requires two nuclear divisions Chromosomes synapse and cross over Halves chromosome number Produces four daughter nuclei Produces daughter cells genetically different from parent and each other Used only for sexual reproduction Mitosis Requires one nuclear division Chromosomes do not synapse nor cross over Preserves chromosome number Produces two daughter nuclei Produces daughter cells genetically identical to parent and to each other Used for asexual reproduction, growth, development, and repair Meiosis versus Mitosis

26. Comparison of Mitosis and Meiosis

27. Sexual reproduction increases genetic variation through three key mechanisms 1. Crossing over 2. Independent assortment 3. Random fertilization Evolutionary Consequences of Sex

28. Random Fertilization • The zygote is formed by the union of two independently-produced gametes • Therefore, the possible combinations in an offspring • 8,388,608 X 8,388,608 = • 70,368,744,177,664 • More than 70 trillion! • And this number does not count crossing-over

30. Genetic Variation: Significance • Asexual reproduction produces genetically identical clones • Asexual reproduction is advantageous when environment is stable • Sexual reproduction produces genetically unique combinations • However, if environment changes, genetic variability introduced by sexual reproduction may be advantageous • Genetic diversity is the raw material that fuels evolution

31. The Human Life Cycle • Requires both mitosis and meiosis • The formation of gametes (eggs and sperm) is called gametogenesis. • In females meiosis is part of the process of oogenesis • In males meiosis is part of spermatogenesis • At fertilization, the resulting zygote divides by mitosis for the processes of growth and development • Mitosis is used for repair throughout life

32. Life Cycle of Humans

33. Spermatogenesis • Begins at puberty and continues throughout life • Occurs in seminiferous tubules of testes • Primary spermatocytes (2n) divide in meiosis I to form 2 secondary spermatocytes (1n) • Secondary spermatocytes divide in meiosis II to produce 4 sperm

34. Oogenesis • Occurs in the ovaries • Primary oocyte (2n) divides in meiosis I to produce 1 secondary oocyte (1n) and 1 polar body • Division is unequal as secondary oocyte receives most of the cell contents (nearly all cytoplasm and organelles) and half the chromosomes • Allows ovum to have all the cellular “machinery” it needs for embryonic development • Polar body functions only to receive half of the chromosomes • Secondary oocyte begins meiosis II but stops at metaphase II; polar body may also divide • At puberty, after ovulation secondary oocyte is activated if fertilized to complete division • Meiosis II produces 1 ovum and up to 3 polar bodies

35. Spermatogenesis and Oogenesis

36. Summary • Spermatogenesis and oogenesis both utilize meiosis • Spermatogenesis begins at puberty and continues throughout life • Spermatogenesis produces 4 sperm per primary spermatocyte • Results in production of many sperm • Oogenesis results in 1 oocyte and up to 3 polar bodies per primary oocyte • Divisions are unequal, ovum receives most cell contents • Oogenesis begins prior to birth, stops until puberty, then resumes in a cyclic pattern • Cyclic release of oocytes continues until menopause when the process stops

37. Human Chromosomes • Human somatic cells have 23 pairs of chromosomes • 22 pairs of autosomes • Autosome-any chromosome other than a sex chromosome • 1 pair of sex chromosomes • XX in females • XY in males

38. Human Chromosomes • Failure of chromosomes to separate correctly in meiosis I or II is termed nondisjunction. • This leads to an abnormal number of chromosomes, or aneuploidy. • Humans with one less autosome are called monosomics. • These do not survive development. • Humans with one extra autosome are called trisomics. • The vast majority do not survive • Trisomy for only a few chromosomes is compatible with survival • However, there are severe developmental defects • The only one compatible with a reasonable chance of survival is trisomy 21 (Down Syndrome).

39. Down Syndrome • Most common trisomy in humans. • Short stature, eyelid fold, flat face, stubby fingers,, round head, mental retardation • 3 copies of chromosome 21 • 75% of cases- egg has 2 copies, sperm has 1 • Can be detected by a karyotype 1 in 1,500 if mother is under 30 1 in 16 if mother is over 45

40. When nondisjunction occurs during meiosis I both members of a homologous pair migrate into the same daughter cell. When nondisjunction occurs in meiosis II, the centromere fails to divide and both daughter chromatids enter the same gamete. • Egg with 24 chromosomes fertilized by sperm with 23- trisomy • 47 chromosomes in zygote • Egg with 22 chromosomes fertilized by sperm with 23 chromosomes- monosomy • 45 chromosomes in zygote • Normal development depends on the presence of exactly 2 of each kind of chromosome

41. Nondisjunction of Chromosomes During Oogenesis Followed by Fertilization with Normal Sperm

42. Nondisjunction Involving Sex Chromosomes • Aneuploidies of sex chromosomes have less serious consequences than those of autosomes • Chances of survival are greatest if monosomy or trisomy involves the sex chromosomes • However, they can lead to sterility

43. Nondisjunction of the X Chromosome

44. Syndrome-disorders characterized by groups of symptoms. Turner’s syndrome • Monosomy X (XO), zygote has one X chromosome and no other X or Y • Capable of survival, phenotypically female, infertile • Ovarian failure Klinefelter syndrome • XXY • Underdeveloped testes and prostate gland, no facial hair • Phenotypically male, infertile Poly-X females • More than 2 X chromosomes • XXX females may be unusually tall • XXXX females are usually severely retarded

45. Turner and Klinefelter Syndromes

46. Nondisjunction of the Y chromosome Jacob’s syndrome • Due to nondisjunction in meiosis II • Yields YY gametes and ultimately XYY zygotes • XYY genotype can only result from nondisjunction in spermatogenesis • Frequency of XYY is 1 in 800 males (live births) • In general, these individuals are phenotypically normal • Taller than average, persistent acne

47. Syndromes from Abnormal Chromosome Numbers