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Novel Protein design

Novel Protein design. BTY516: protein engineering . Question. Why design novel proteins? what can be gained by the design and characterization of novel proteins? Can we design 3D protein structures that have never been observed?

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Novel Protein design

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  1. Novel Protein design BTY516: protein engineering

  2. Question • Why design novel proteins? • what can be gained by the design and characterization of novel proteins? • Can we design 3D protein structures that have never been observed? • Are the 3D structures that have not been observed in nature unattainable, or just unsampled?

  3. Main Motivations • our understanding of natural proteins--their folding pathways, thermodynamic stabilities, and catalytic properties -- is enhanced (and ultimately tested) by our ability to design novel proteins with predetermined structures and properties. • For solving important chemical or biochemical problems.

  4. STRATEGIES FOR THE DESIGN OF STRUCTURE • Self-Assembly of Modular Units of Secondary Structure End-on view of two parallel (α-helices forming a coiled-coil structure. A single heptad repeat is shown and the view is looking from the N-terminal end with the a-helices descending into the page. Schematic diagram showing the self-assembly of two peptides into an ordered dimeric structure.

  5. STRATEGIES FOR THE DESIGN OF STRUCTURE Schematic illustration of the incremental approach

  6. STRATEGIES FOR THE DESIGN OF STRUCTURE 2- Ligand-lnduced Assembly Ligand-induced assembly of a three-helix bundle.

  7. STRATEGIES FOR THE DESIGN OF STRUCTURE 2- Ligand-lnduced Assembly Schematic diagram showing ligand-induced assembly.

  8. STRATEGIES FOR THE DESIGN OF STRUCTURE 3- Assembly of Peptides via Covalent Cross-linking Schematic diagram showing the assembly of a structure that is stabilized by covalent cross-link

  9. STRATEGIES FOR THE DESIGN OF STRUCTURE The amino acid sequence and cross-linking pattern of Chymohelizyme

  10. STRATEGIES FOR THE DESIGN OF STRUCTURE 4- Assembly of Peptides on a Synthetic Template Schematic diagram of a template-assembled synthetic protein (TASP).

  11. STRATEGIES FOR THE DESIGN OF STRUCTURE 5-Linear Polypeptides That Fold into Globular Structures Ribbon drawing of the sequence and proposed structure of Felix. The Cysll-Cys71 disulfide bond is indicated

  12. Reference Carey, P.R. (1996). Protein Engineering and Design. Academic Press.

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