70 likes | 263 Views
LB Broth Evaluation prior Bacteria Inoculation Media Quality Control. LB Broth Evaluation. Volume It should be between 45-50ml Heating will cause evaporation 10% rule allows liquid loss How to score Happy face 45-50ml Sad face 45ml and below. LB Broth Evaluation . Color
E N D
LB Broth Evaluationprior Bacteria Inoculation Media Quality Control
LB Broth Evaluation Volume It should be between 45-50ml Heating will cause evaporation 10% rule allows liquid loss How to score Happy face 45-50ml Sad face 45ml and below
LB Broth Evaluation Color Should be a straw or golden brown color Black flakes due to boiling too much Dark brown due to too much broth How to score Happy face Golden Brown Sad face Black flakes or dark brown
LB Broth Evaluation Mix No clumps- consistent How to score Happy face No clumps Sad face Clumps
Media Evaluation Record your observations in your lab book Make sure caps are tight Start your lab and follow the procedures