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PolExGene meeting december, 17-18 2007 Ghent

PolExGene meeting december, 17-18 2007 Ghent. CNRS Montpellier (France), Partner 10 Max Mousseron Institute of Biomolecules Pr. Jean Martinez Dr. Muriel Amblard Dr. Gilles Subra Dr. Jean-Alain Fehrentz Luc Brunel. WP 2 : Synthesis of Selected CPP. WP 3 : Synthesis of Selected CIP.

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PolExGene meeting december, 17-18 2007 Ghent

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  1. PolExGene meeting december, 17-18 2007 Ghent CNRS Montpellier (France), Partner 10 Max Mousseron Institute of Biomolecules Pr. Jean Martinez Dr. Muriel Amblard Dr. Gilles Subra Dr. Jean-Alain Fehrentz Luc Brunel WP 2 : Synthesis of Selected CPP WP 3 : Synthesis of Selected CIP

  2. Objectives of our group Selection and synthesis of Cell Penetrating Peptides (CPP) Increase internalisation for DNA-delivery CPP-containing polyplexes (covalent anchoring) CPP + polyplexes (mixtures) Selection and synthesis of Cell Interacting Peptides (CIP) Increase membrane-cell interaction

  3. Cell Penetrating Peptide Synthesis Peptides deriving from protein transduction domain Tat Partn n° Tat-NH2 H-GRKKRRQRRRPPQ-NH2 JMV3535 5 Acetyl-Tat-NH2 Ac-GRKKRRQRRRPPQ-NH2 JMV3536 5 Ac-GRKKRRQRRR-NH2 Acetyl-Tat(48-57)-NH2 JMV3484 5 Tat(48-57) H-GRKKRRQRRR-OH JMV3481 5 Tat(Arg3,4) H-GRRRRRQRRRPPQ-OH JMV3508 5 Ac-Tat(Arg3,4) Ac-GRRRRRQRRRPPQ-OH JMV3509 5

  4. Cell Penetrating Peptide Synthesis Polyarginine-based peptides Partn n° Ac-(Arg)8-OH Ac-RRRRRRRR-OH JMV3482 5 Ac-(Arg)11-OH Ac-RRRRRRRRRRR-OH JMV3483 5 H-(Arg)8-NH2 H-RRRRRRRR-NH2 JMV3506 5 Ac-RRRRRRRR-NH2 Ac-(Arg)8-NH2 JMV3507 5 Biotine-RRRRRRRR-NH2 Biotine-(Arg)8-NH2 JMV3674 8

  5. Tagged Cell Penetrating Peptide • Tagged CPP N-ter, C-ter or within chain (FTIC, others?, Qdot?) RhodB-Penetratin Rho-RQIKIWFQNRRMKWKK-OH Partner 3 (IBMT) Biotine-RRRRRRRR-NH2 Biotine-(Arg)8-NH2 Partner 8 (ARK)

  6. KALA Translocating Peptide Dr Jani Räty (Ark Therapeutics, Partner number 8) KALA peptide - 30 amino acid synthetic peptide, cationic amphipathic peptide - enhances gene delivery Biotine-KALA Biotine-WEAKLAKALAKALAKHLAKALAKALKACEA JMV3690

  7. General Strategy of Synthesis

  8. Peptide synthesis and purification Manual synthesiser Advanced Chemtech ACT496 synthesiser Waters LC/MS autopurification

  9. Cell Interacting Peptide Synthesis RGD-based peptides (avb3 integrin) Partn n° H-Cys-Gly-Gly-Arg-Gly-Asp-Ser-OH JMV3622 1 cyclo[Arg-Gly-Asp-(D)Phe-Lysw](H-Cys-Gly-Gly-) JMV3619 1 Integrin: Cell surface receptor that mediates adhesion between cells and extracellular matrix

  10. JMV3619 Synthesis

  11. Needs of PolExGene Partners CPP and CIP Type (Tat, Arg, penetratin, others?) Other specific CIPs ? Fonctionnalisation?

  12. Perspectives • Modified CPP Lead CPP (Penetratin, Tat or polyarginine) Bioavailability : synthesis of lead analogues like : D-peptides beta-peptides Peptoids • Selection of CIP Specific sequences for specific receptors: other than RGD?

  13. Cell Penetrating Peptide for covalent anchoringon cationic polymers Nter Cys peptides for maleimide ligation or SS bond Ac-Cys-RQIKIWFQNRRMKWKK-OH Ac-Cys-GRKKRRQRRRPPQ-OH Rem: Cys can be replaced by simpler thiol containg group Ac-Cys-RRRRRRRR-OH Cter Cys peptides for maleimide ligation or SS bond Ac-RQIKIWFQNRRMKWKK-Cys-OH Ac-GRKKRRQRRRPPQ-Cys-OH Ac-RRRRRRRR-Cys-OH

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